Strain Fitness in Escherichia coli BW25113 around b2405

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgltX and valU are separated by 258 nucleotidesvalU and valX are separated by 44 nucleotidesvalX and valY are separated by 46 nucleotidesvalY and lysV are separated by 4 nucleotideslysV and xapR are separated by 264 nucleotidesxapR and xapB are separated by 251 nucleotides b2400: gltX - glutamyl-tRNA synthetase (NCBI), at 2,517,279 to 2,518,694 gltX b2401: valU - tRNA-Val (NCBI), at 2,518,953 to 2,519,028 valU b2402: valX - tRNA-Val (NCBI), at 2,519,073 to 2,519,148 valX b2403: valY - tRNA-Val (NCBI), at 2,519,195 to 2,519,270 valY b2404: lysV - tRNA-Lys (NCBI), at 2,519,275 to 2,519,350 lysV b2405: xapR - DNA-binding transcriptional activator (NCBI), at 2,519,615 to 2,520,499 xapR b2406: xapB - xanthosine transporter (NCBI), at 2,520,751 to 2,522,007 xapB Position (kb) 2519 2520 2521Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2518.799 kb on + strandat 2518.935 kb on - strandat 2518.935 kb on - strandat 2518.939 kb on + strandat 2519.620 kb on - strandat 2519.657 kb on + strandat 2519.665 kb on - strandat 2519.679 kb on + strandat 2519.687 kb on - strandat 2519.788 kb on + strand, within xapRat 2519.788 kb on + strand, within xapRat 2519.891 kb on - strand, within xapRat 2519.891 kb on - strand, within xapRat 2519.891 kb on - strand, within xapRat 2519.970 kb on - strand, within xapRat 2520.091 kb on - strand, within xapRat 2520.091 kb on - strand, within xapRat 2520.181 kb on - strand, within xapRat 2520.188 kb on - strand, within xapRat 2520.195 kb on - strand, within xapRat 2520.207 kb on - strand, within xapRat 2520.208 kb on + strand, within xapRat 2520.208 kb on + strand, within xapRat 2520.224 kb on + strand, within xapRat 2520.224 kb on + strand, within xapRat 2520.245 kb on - strand, within xapRat 2520.288 kb on + strand, within xapRat 2520.303 kb on + strand, within xapRat 2520.311 kb on + strand, within xapRat 2520.311 kb on + strand, within xapRat 2520.313 kb on + strand, within xapRat 2520.313 kb on + strand, within xapRat 2520.313 kb on + strand, within xapRat 2520.313 kb on + strand, within xapRat 2520.324 kb on + strand, within xapRat 2520.336 kb on + strand, within xapRat 2520.336 kb on + strand, within xapRat 2520.337 kb on + strand, within xapRat 2520.344 kb on - strand, within xapRat 2520.454 kb on + strandat 2520.454 kb on + strandat 2520.470 kb on + strandat 2520.480 kb on + strandat 2520.485 kb on + strandat 2520.485 kb on + strandat 2520.485 kb on + strandat 2520.648 kb on - strandat 2520.734 kb on - strandat 2520.734 kb on - strandat 2520.739 kb on - strandat 2520.770 kb on - strandat 2520.863 kb on - strandat 2520.863 kb on - strandat 2520.902 kb on + strand, within xapBat 2520.921 kb on - strand, within xapBat 2520.932 kb on - strand, within xapBat 2520.932 kb on - strand, within xapBat 2520.949 kb on - strand, within xapBat 2520.964 kb on - strand, within xapBat 2520.964 kb on - strand, within xapBat 2521.099 kb on - strand, within xapBat 2521.141 kb on - strand, within xapBat 2521.141 kb on - strand, within xapBat 2521.141 kb on - strand, within xapBat 2521.141 kb on - strand, within xapBat 2521.151 kb on - strand, within xapBat 2521.303 kb on - strand, within xapBat 2521.359 kb on - strand, within xapBat 2521.359 kb on - strand, within xapBat 2521.437 kb on - strand, within xapB

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,518,799 + -1.1
2,518,935 - +0.1
2,518,935 - -1.1
2,518,939 + -1.3
2,519,620 - +1.5
2,519,657 + +0.8
2,519,665 - +2.2
2,519,679 + -1.9
2,519,687 - -0.7
2,519,788 + xapR b2405 0.20 -0.5
2,519,788 + xapR b2405 0.20 -0.2
2,519,891 - xapR b2405 0.31 +0.3
2,519,891 - xapR b2405 0.31 -1.1
2,519,891 - xapR b2405 0.31 -0.1
2,519,970 - xapR b2405 0.40 +0.2
2,520,091 - xapR b2405 0.54 -0.8
2,520,091 - xapR b2405 0.54 +0.3
2,520,181 - xapR b2405 0.64 -0.7
2,520,188 - xapR b2405 0.65 -1.1
2,520,195 - xapR b2405 0.66 +0.7
2,520,207 - xapR b2405 0.67 +0.6
2,520,208 + xapR b2405 0.67 -0.3
2,520,208 + xapR b2405 0.67 -0.5
2,520,224 + xapR b2405 0.69 +0.5
2,520,224 + xapR b2405 0.69 +1.1
2,520,245 - xapR b2405 0.71 -1.1
2,520,288 + xapR b2405 0.76 +0.7
2,520,303 + xapR b2405 0.78 +0.6
2,520,311 + xapR b2405 0.79 +1.2
2,520,311 + xapR b2405 0.79 +2.0
2,520,313 + xapR b2405 0.79 +1.8
2,520,313 + xapR b2405 0.79 +0.7
2,520,313 + xapR b2405 0.79 +0.4
2,520,313 + xapR b2405 0.79 +0.7
2,520,324 + xapR b2405 0.80 -0.2
2,520,336 + xapR b2405 0.81 -0.8
2,520,336 + xapR b2405 0.81 -0.8
2,520,337 + xapR b2405 0.82 -0.5
2,520,344 - xapR b2405 0.82 +1.0
2,520,454 + -0.3
2,520,454 + -0.4
2,520,470 + -1.5
2,520,480 + -3.4
2,520,485 + -1.7
2,520,485 + +0.5
2,520,485 + +0.2
2,520,648 - -2.7
2,520,734 - +0.1
2,520,734 - +1.4
2,520,739 - -1.2
2,520,770 - +1.4
2,520,863 - +0.8
2,520,863 - -1.1
2,520,902 + xapB b2406 0.12 -1.3
2,520,921 - xapB b2406 0.14 -1.0
2,520,932 - xapB b2406 0.14 +0.1
2,520,932 - xapB b2406 0.14 +1.7
2,520,949 - xapB b2406 0.16 +1.2
2,520,964 - xapB b2406 0.17 +2.3
2,520,964 - xapB b2406 0.17 -0.2
2,521,099 - xapB b2406 0.28 -1.2
2,521,141 - xapB b2406 0.31 +0.1
2,521,141 - xapB b2406 0.31 -0.8
2,521,141 - xapB b2406 0.31 -0.5
2,521,141 - xapB b2406 0.31 -0.0
2,521,151 - xapB b2406 0.32 -0.8
2,521,303 - xapB b2406 0.44 +1.8
2,521,359 - xapB b2406 0.48 +0.2
2,521,359 - xapB b2406 0.48 -0.5
2,521,437 - xapB b2406 0.55 -0.4

Or see this region's nucleotide sequence