Strain Fitness in Escherichia coli BW25113 around b1939

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliF and fliG overlap by 8 nucleotidesfliG and fliH overlap by 8 nucleotidesfliH and fliI overlap by 1 nucleotides b1938: fliF - flagellar M-ring protein (NCBI), at 2,011,253 to 2,012,911 fliF b1939: fliG - flagellar motor switch protein G (NCBI), at 2,012,904 to 2,013,899 fliG b1940: fliH - flagellar biosynthesis; export of flagellar proteins? (VIMSS), at 2,013,892 to 2,014,578 fliH b1941: fliI - flagellum-specific ATP synthase (NCBI), at 2,014,578 to 2,015,951 fliI Position (kb) 2012 2013 2014Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2012.056 kb on - strand, within fliFat 2012.086 kb on + strand, within fliFat 2012.086 kb on + strand, within fliFat 2012.086 kb on + strand, within fliFat 2012.143 kb on - strand, within fliFat 2012.143 kb on - strand, within fliFat 2012.165 kb on - strand, within fliFat 2012.208 kb on - strand, within fliFat 2012.208 kb on - strand, within fliFat 2012.276 kb on + strand, within fliFat 2012.281 kb on + strand, within fliFat 2012.281 kb on + strand, within fliFat 2012.443 kb on - strand, within fliFat 2012.523 kb on - strand, within fliFat 2012.812 kb on - strandat 2012.830 kb on + strandat 2012.865 kb on + strandat 2012.873 kb on - strandat 2012.922 kb on + strandat 2012.979 kb on + strandat 2012.979 kb on + strandat 2013.108 kb on + strand, within fliGat 2013.108 kb on + strand, within fliGat 2013.149 kb on - strand, within fliGat 2013.444 kb on + strand, within fliGat 2014.036 kb on + strand, within fliHat 2014.099 kb on - strand, within fliHat 2014.120 kb on + strand, within fliHat 2014.214 kb on + strand, within fliHat 2014.240 kb on - strand, within fliHat 2014.246 kb on + strand, within fliHat 2014.318 kb on - strand, within fliHat 2014.336 kb on + strand, within fliHat 2014.404 kb on - strand, within fliHat 2014.562 kb on + strandat 2014.562 kb on + strandat 2014.829 kb on + strand, within fliIat 2014.829 kb on + strand, within fliI

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,012,056 - fliF b1938 0.48 -2.3
2,012,086 + fliF b1938 0.50 -2.9
2,012,086 + fliF b1938 0.50 -2.7
2,012,086 + fliF b1938 0.50 -0.7
2,012,143 - fliF b1938 0.54 -1.8
2,012,143 - fliF b1938 0.54 -1.9
2,012,165 - fliF b1938 0.55 -1.6
2,012,208 - fliF b1938 0.58 -1.6
2,012,208 - fliF b1938 0.58 -2.0
2,012,276 + fliF b1938 0.62 -2.1
2,012,281 + fliF b1938 0.62 -0.2
2,012,281 + fliF b1938 0.62 -2.1
2,012,443 - fliF b1938 0.72 -1.5
2,012,523 - fliF b1938 0.77 -1.8
2,012,812 - -1.2
2,012,830 + -2.7
2,012,865 + -1.2
2,012,873 - -0.1
2,012,922 + -0.6
2,012,979 + -4.3
2,012,979 + -2.3
2,013,108 + fliG b1939 0.20 +0.1
2,013,108 + fliG b1939 0.20 -1.3
2,013,149 - fliG b1939 0.25 -2.2
2,013,444 + fliG b1939 0.54 -3.7
2,014,036 + fliH b1940 0.21 -3.3
2,014,099 - fliH b1940 0.30 -1.1
2,014,120 + fliH b1940 0.33 -1.9
2,014,214 + fliH b1940 0.47 -2.4
2,014,240 - fliH b1940 0.51 -3.2
2,014,246 + fliH b1940 0.52 -0.7
2,014,318 - fliH b1940 0.62 -2.2
2,014,336 + fliH b1940 0.65 -1.5
2,014,404 - fliH b1940 0.75 +0.4
2,014,562 + -1.6
2,014,562 + -1.8
2,014,829 + fliI b1941 0.18 +0.2
2,014,829 + fliI b1941 0.18 -2.6

Or see this region's nucleotide sequence