Strain Fitness in Escherichia coli BW25113 around b1853

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntzwf and yebK are separated by 337 nucleotidesyebK and pykA are separated by 127 nucleotides b1852: zwf - glucose-6-phosphate 1-dehydrogenase (NCBI), at 1,932,863 to 1,934,338 zwf b1853: yebK - predicted DNA-binding transcriptional regulator (NCBI), at 1,934,676 to 1,935,545 yebK b1854: pykA - pyruvate kinase (NCBI), at 1,935,673 to 1,937,115 pykA Position (kb) 1934 1935 1936Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1934.272 kb on + strandat 1934.305 kb on + strandat 1934.347 kb on - strandat 1934.347 kb on - strandat 1934.373 kb on + strandat 1934.409 kb on + strandat 1934.587 kb on + strandat 1934.587 kb on + strandat 1934.818 kb on + strand, within yebKat 1934.893 kb on + strand, within yebKat 1935.346 kb on - strand, within yebKat 1935.359 kb on + strand, within yebKat 1935.869 kb on - strand, within pykAat 1935.871 kb on + strand, within pykAat 1935.919 kb on - strand, within pykAat 1935.919 kb on - strand, within pykAat 1935.962 kb on + strand, within pykAat 1935.962 kb on + strand, within pykAat 1935.979 kb on + strand, within pykAat 1936.028 kb on + strand, within pykAat 1936.028 kb on + strand, within pykAat 1936.223 kb on - strand, within pykAat 1936.241 kb on - strand, within pykAat 1936.241 kb on - strand, within pykAat 1936.292 kb on - strand, within pykAat 1936.299 kb on - strand, within pykAat 1936.412 kb on - strand, within pykA

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,934,272 + -2.7
1,934,305 + -1.1
1,934,347 - +0.2
1,934,347 - +0.1
1,934,373 + +0.4
1,934,409 + -1.0
1,934,587 + +1.3
1,934,587 + +1.1
1,934,818 + yebK b1853 0.16 +1.4
1,934,893 + yebK b1853 0.25 +3.7
1,935,346 - yebK b1853 0.77 -1.9
1,935,359 + yebK b1853 0.79 +0.0
1,935,869 - pykA b1854 0.14 -3.5
1,935,871 + pykA b1854 0.14 +0.8
1,935,919 - pykA b1854 0.17 +0.3
1,935,919 - pykA b1854 0.17 -0.5
1,935,962 + pykA b1854 0.20 -1.7
1,935,962 + pykA b1854 0.20 -1.9
1,935,979 + pykA b1854 0.21 +0.2
1,936,028 + pykA b1854 0.25 -0.4
1,936,028 + pykA b1854 0.25 +0.2
1,936,223 - pykA b1854 0.38 +0.7
1,936,241 - pykA b1854 0.39 -1.1
1,936,241 - pykA b1854 0.39 -1.2
1,936,292 - pykA b1854 0.43 +0.2
1,936,299 - pykA b1854 0.43 -0.3
1,936,412 - pykA b1854 0.51 -1.6

Or see this region's nucleotide sequence