Strain Fitness in Escherichia coli BW25113 around b1285

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciT and yciZ are separated by 89 nucleotidesyciZ and gmr are separated by 147 nucleotidesgmr and ymiB are separated by 53 nucleotidesymiB and rnb are separated by 77 nucleotides b1284: yciT - predicted DNA-binding transcriptional regulator (NCBI), at 1,341,621 to 1,342,370 yciT b4596: yciZ - hypothetical protein (RefSeq), at 1,342,460 to 1,342,633 yciZ b1285: gmr - modulator of Rnase II stability (NCBI), at 1,342,781 to 1,344,766 gmr b4672: ymiB - expressed protein (RefSeq), at 1,344,820 to 1,344,924 ymiB b1286: rnb - exoribonuclease II (NCBI), at 1,345,002 to 1,346,936 rnb Position (kb) 1342 1343 1344 1345Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1341.823 kb on - strand, within yciTat 1342.104 kb on + strand, within yciTat 1342.104 kb on + strand, within yciTat 1342.216 kb on - strand, within yciTat 1342.306 kb on - strandat 1342.313 kb on + strandat 1342.313 kb on + strandat 1342.329 kb on + strandat 1342.478 kb on + strand, within yciZat 1342.478 kb on + strand, within yciZat 1342.708 kb on - strandat 1342.809 kb on - strandat 1343.191 kb on - strand, within gmrat 1343.224 kb on + strand, within gmrat 1343.224 kb on + strand, within gmrat 1343.235 kb on + strand, within gmrat 1343.621 kb on + strand, within gmrat 1343.621 kb on + strand, within gmrat 1343.700 kb on - strand, within gmrat 1343.705 kb on + strand, within gmrat 1343.740 kb on + strand, within gmrat 1343.840 kb on + strand, within gmrat 1343.849 kb on + strand, within gmrat 1343.988 kb on + strand, within gmrat 1343.988 kb on + strand, within gmrat 1344.018 kb on + strand, within gmrat 1344.084 kb on - strand, within gmrat 1344.111 kb on + strand, within gmrat 1344.215 kb on + strand, within gmrat 1344.256 kb on - strand, within gmrat 1344.290 kb on - strand, within gmrat 1344.481 kb on + strand, within gmrat 1345.121 kb on + strandat 1345.306 kb on - strand, within rnbat 1345.321 kb on - strand, within rnbat 1345.321 kb on - strand, within rnbat 1345.492 kb on - strand, within rnbat 1345.550 kb on - strand, within rnbat 1345.727 kb on + strand, within rnbat 1345.736 kb on - strand, within rnb

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,341,823 - yciT b1284 0.27 +2.2
1,342,104 + yciT b1284 0.64 +0.8
1,342,104 + yciT b1284 0.64 +2.3
1,342,216 - yciT b1284 0.79 +3.4
1,342,306 - +0.4
1,342,313 + +1.1
1,342,313 + -1.5
1,342,329 + +1.7
1,342,478 + yciZ b4596 0.10 +1.2
1,342,478 + yciZ b4596 0.10 -0.2
1,342,708 - -0.5
1,342,809 - -0.9
1,343,191 - gmr b1285 0.21 -3.3
1,343,224 + gmr b1285 0.22 +1.4
1,343,224 + gmr b1285 0.22 +1.0
1,343,235 + gmr b1285 0.23 +2.2
1,343,621 + gmr b1285 0.42 +1.0
1,343,621 + gmr b1285 0.42 -0.2
1,343,700 - gmr b1285 0.46 -0.8
1,343,705 + gmr b1285 0.47 -0.3
1,343,740 + gmr b1285 0.48 -0.2
1,343,840 + gmr b1285 0.53 +0.4
1,343,849 + gmr b1285 0.54 -1.6
1,343,988 + gmr b1285 0.61 +0.2
1,343,988 + gmr b1285 0.61 +1.0
1,344,018 + gmr b1285 0.62 +0.6
1,344,084 - gmr b1285 0.66 +1.7
1,344,111 + gmr b1285 0.67 -0.1
1,344,215 + gmr b1285 0.72 +1.6
1,344,256 - gmr b1285 0.74 -2.3
1,344,290 - gmr b1285 0.76 -0.4
1,344,481 + gmr b1285 0.86 -0.9
1,345,121 + -1.5
1,345,306 - rnb b1286 0.16 -2.1
1,345,321 - rnb b1286 0.16 -1.3
1,345,321 - rnb b1286 0.16 -2.4
1,345,492 - rnb b1286 0.25 -1.4
1,345,550 - rnb b1286 0.28 -2.1
1,345,727 + rnb b1286 0.37 -2.2
1,345,736 - rnb b1286 0.38 -2.4

Or see this region's nucleotide sequence