Strain Fitness in Escherichia coli BW25113 around b1277

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntacnA and ribA are separated by 63 nucleotidesribA and pgpB are separated by 169 nucleotides b1276: acnA - aconitate hydratase (NCBI), at 1,333,855 to 1,336,530 acnA b1277: ribA - GTP cyclohydrolase II protein (NCBI), at 1,336,594 to 1,337,184 ribA b1278: pgpB - phosphatidylglycerophosphatase B (NCBI), at 1,337,354 to 1,338,118 pgpB Position (kb) 1336 1337 1338Strain fitness (log2 ratio) -2 -1 0 1 2at 1335.601 kb on - strand, within acnAat 1335.601 kb on - strand, within acnAat 1335.669 kb on + strand, within acnAat 1335.669 kb on + strand, within acnAat 1335.825 kb on - strand, within acnAat 1335.825 kb on - strand, within acnAat 1335.902 kb on - strand, within acnAat 1335.902 kb on - strand, within acnAat 1336.129 kb on - strand, within acnAat 1336.223 kb on + strand, within acnAat 1337.219 kb on - strandat 1337.219 kb on - strandat 1337.498 kb on + strand, within pgpBat 1337.503 kb on + strand, within pgpBat 1337.522 kb on - strand, within pgpBat 1338.069 kb on - strandat 1338.069 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,335,601 - acnA b1276 0.65 +0.2
1,335,601 - acnA b1276 0.65 -0.9
1,335,669 + acnA b1276 0.68 +1.2
1,335,669 + acnA b1276 0.68 +1.2
1,335,825 - acnA b1276 0.74 -0.8
1,335,825 - acnA b1276 0.74 +0.4
1,335,902 - acnA b1276 0.76 +0.9
1,335,902 - acnA b1276 0.76 -0.1
1,336,129 - acnA b1276 0.85 +1.2
1,336,223 + acnA b1276 0.88 +1.1
1,337,219 - -2.0
1,337,219 - +1.6
1,337,498 + pgpB b1278 0.19 +0.0
1,337,503 + pgpB b1278 0.19 +0.2
1,337,522 - pgpB b1278 0.22 +1.8
1,338,069 - +1.7
1,338,069 - +0.4

Or see this region's nucleotide sequence