Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mviN and flgN are separated by 39 nucleotides flgN and flgM are separated by 4 nucleotides flgM and flgA are separated by 75 nucleotides flgA and flgB are separated by 154 nucleotides flgB and flgC are separated by 3 nucleotides flgC and flgD are separated by 11 nucleotides
b1069: mviN - predicted inner membrane protein (NCBI), at 1,127,062 to 1,128,597
mviN
b1070: flgN - export chaperone for FlgK and FlgL (NCBI), at 1,128,637 to 1,129,053
flgN
b1071: flgM - anti-sigma factor for FliA (sigma 28) (NCBI), at 1,129,058 to 1,129,351
flgM
b1072: flgA - flagellar basal body P-ring biosynthesis protein A (NCBI), at 1,129,427 to 1,130,086
flgA
b1073: flgB - flagellar basal-body rod protein B (NCBI), at 1,130,241 to 1,130,657
flgB
b1074: flgC - flagellar basal-body rod protein C (NCBI), at 1,130,661 to 1,131,065
flgC
b1075: flgD - flagellar basal body rod modification protein D (NCBI), at 1,131,077 to 1,131,772
flgD
Position (kb)
1129
1130
1131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1128.553 kb on + strand at 1128.592 kb on + strand at 1128.600 kb on - strand at 1128.612 kb on + strand at 1128.932 kb on - strand, within flgN at 1129.057 kb on + strand at 1129.094 kb on - strand, within flgM at 1129.303 kb on + strand, within flgM at 1129.414 kb on - strand at 1129.433 kb on - strand at 1129.433 kb on - strand at 1129.579 kb on + strand, within flgA at 1129.663 kb on + strand, within flgA at 1129.716 kb on + strand, within flgA at 1129.771 kb on + strand, within flgA at 1129.771 kb on + strand, within flgA at 1129.810 kb on + strand, within flgA at 1129.811 kb on - strand, within flgA at 1129.813 kb on + strand, within flgA at 1129.815 kb on + strand, within flgA at 1129.815 kb on + strand, within flgA at 1129.958 kb on + strand, within flgA at 1130.045 kb on + strand at 1130.097 kb on + strand at 1130.110 kb on + strand at 1130.161 kb on + strand at 1130.236 kb on - strand at 1130.238 kb on - strand at 1130.238 kb on - strand at 1130.270 kb on - strand at 1130.352 kb on - strand, within flgB at 1130.352 kb on - strand, within flgB at 1130.411 kb on + strand, within flgB at 1130.411 kb on + strand, within flgB at 1130.456 kb on - strand, within flgB at 1130.456 kb on - strand, within flgB at 1130.513 kb on - strand, within flgB at 1130.570 kb on - strand, within flgB at 1130.693 kb on - strand at 1130.701 kb on + strand at 1131.014 kb on + strand, within flgC at 1131.014 kb on + strand, within flgC at 1131.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,128,553 + +0.7 1,128,592 + +0.4 1,128,600 - -0.3 1,128,612 + +1.6 1,128,932 - flgN b1070 0.71 +0.3 1,129,057 + -1.4 1,129,094 - flgM b1071 0.12 +0.8 1,129,303 + flgM b1071 0.83 +0.5 1,129,414 - +0.3 1,129,433 - +0.7 1,129,433 - -1.2 1,129,579 + flgA b1072 0.23 +0.4 1,129,663 + flgA b1072 0.36 -1.1 1,129,716 + flgA b1072 0.44 -1.9 1,129,771 + flgA b1072 0.52 +0.3 1,129,771 + flgA b1072 0.52 -1.8 1,129,810 + flgA b1072 0.58 -3.1 1,129,811 - flgA b1072 0.58 +0.1 1,129,813 + flgA b1072 0.58 -1.4 1,129,815 + flgA b1072 0.59 -0.7 1,129,815 + flgA b1072 0.59 -1.9 1,129,958 + flgA b1072 0.80 -3.2 1,130,045 + -0.6 1,130,097 + -1.5 1,130,110 + +0.4 1,130,161 + -0.3 1,130,236 - -1.3 1,130,238 - -2.3 1,130,238 - -1.8 1,130,270 - -1.0 1,130,352 - flgB b1073 0.27 -1.0 1,130,352 - flgB b1073 0.27 -3.3 1,130,411 + flgB b1073 0.41 -3.1 1,130,411 + flgB b1073 0.41 -0.5 1,130,456 - flgB b1073 0.52 -3.3 1,130,456 - flgB b1073 0.52 +0.0 1,130,513 - flgB b1073 0.65 -1.9 1,130,570 - flgB b1073 0.79 -2.6 1,130,693 - -2.3 1,130,701 + -3.5 1,131,014 + flgC b1074 0.87 +1.9 1,131,014 + flgC b1074 0.87 -1.3 1,131,054 - -3.2
Or see this region's nucleotide sequence