Strain Fitness in Escherichia coli BW25113 around b0797

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybhG and ybiH overlap by 1 nucleotidesybiH and rhlE are separated by 228 nucleotidesrhlE and ybiA are separated by 231 nucleotidesybiA and dinG are separated by 119 nucleotides b0795: ybhG - hypothetical protein (NCBI), at 828,197 to 829,195 ybhG b0796: ybiH - putative transcriptional regulator (VIMSS), at 829,195 to 829,866 ybiH b0797: rhlE - RNA helicase (NCBI), at 830,095 to 831,459 rhlE b0798: ybiA - hypothetical protein (NCBI), at 831,691 to 832,173 ybiA b0799: dinG - ATP-dependent DNA helicase (NCBI), at 832,293 to 834,443 dinG Position (kb) 830 831 832Strain fitness (log2 ratio) -2 -1 0 1 2at 829.295 kb on - strand, within ybiHat 829.360 kb on + strand, within ybiHat 829.360 kb on + strand, within ybiHat 829.535 kb on - strand, within ybiHat 829.598 kb on + strand, within ybiHat 829.679 kb on + strand, within ybiHat 829.841 kb on - strandat 829.841 kb on - strandat 829.845 kb on - strandat 829.858 kb on + strandat 829.996 kb on + strandat 830.051 kb on + strandat 830.059 kb on + strandat 830.059 kb on + strandat 830.123 kb on + strandat 830.123 kb on + strandat 830.123 kb on + strandat 830.146 kb on + strandat 830.156 kb on + strandat 830.159 kb on - strandat 830.176 kb on - strandat 830.182 kb on + strandat 830.182 kb on + strandat 830.192 kb on - strandat 830.249 kb on - strand, within rhlEat 830.277 kb on - strand, within rhlEat 830.277 kb on - strand, within rhlEat 830.333 kb on + strand, within rhlEat 830.333 kb on + strand, within rhlEat 830.335 kb on - strand, within rhlEat 830.335 kb on - strand, within rhlEat 830.341 kb on - strand, within rhlEat 830.390 kb on + strand, within rhlEat 830.531 kb on + strand, within rhlEat 830.836 kb on + strand, within rhlEat 830.850 kb on - strand, within rhlEat 830.860 kb on - strand, within rhlEat 830.860 kb on - strand, within rhlEat 831.483 kb on + strandat 831.535 kb on - strandat 831.542 kb on - strandat 831.542 kb on - strandat 831.679 kb on + strandat 831.679 kb on + strandat 831.733 kb on - strandat 831.733 kb on - strandat 831.752 kb on + strand, within ybiAat 831.752 kb on + strand, within ybiAat 831.942 kb on - strand, within ybiAat 832.127 kb on + strandat 832.127 kb on + strandat 832.158 kb on - strandat 832.250 kb on + strandat 832.353 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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829,295 - ybiH b0796 0.15 +2.1
829,360 + ybiH b0796 0.25 -0.9
829,360 + ybiH b0796 0.25 +0.4
829,535 - ybiH b0796 0.51 -0.1
829,598 + ybiH b0796 0.60 +0.0
829,679 + ybiH b0796 0.72 +0.3
829,841 - +1.6
829,841 - +0.8
829,845 - +2.0
829,858 + +1.3
829,996 + +2.5
830,051 + +0.5
830,059 + +1.0
830,059 + -1.0
830,123 + -1.3
830,123 + -1.4
830,123 + +2.0
830,146 + -0.1
830,156 + -0.1
830,159 - -0.4
830,176 - +0.8
830,182 + -1.5
830,182 + -1.7
830,192 - -0.3
830,249 - rhlE b0797 0.11 +2.3
830,277 - rhlE b0797 0.13 -0.7
830,277 - rhlE b0797 0.13 -0.7
830,333 + rhlE b0797 0.17 +0.5
830,333 + rhlE b0797 0.17 -0.5
830,335 - rhlE b0797 0.18 -0.6
830,335 - rhlE b0797 0.18 -2.0
830,341 - rhlE b0797 0.18 +1.0
830,390 + rhlE b0797 0.22 -1.4
830,531 + rhlE b0797 0.32 -0.8
830,836 + rhlE b0797 0.54 -2.2
830,850 - rhlE b0797 0.55 -1.0
830,860 - rhlE b0797 0.56 -0.2
830,860 - rhlE b0797 0.56 -1.7
831,483 + -0.1
831,535 - +1.0
831,542 - +0.2
831,542 - -0.0
831,679 + +0.6
831,679 + +1.8
831,733 - -2.0
831,733 - -0.9
831,752 + ybiA b0798 0.13 +0.7
831,752 + ybiA b0798 0.13 +0.7
831,942 - ybiA b0798 0.52 -2.4
832,127 + -0.8
832,127 + -2.2
832,158 - +0.9
832,250 + +0.9
832,353 - +1.4

Or see this region's nucleotide sequence