Strain Fitness in Escherichia coli BW25113 around b0727

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsucA and sucB are separated by 14 nucleotidessucB and sucC are separated by 274 nucleotides b0726: sucA - alpha-ketoglutarate decarboxylase (NCBI), at 757,929 to 760,730 sucA b0727: sucB - dihydrolipoamide acetyltransferase (NCBI), at 760,745 to 761,962 sucB b0728: sucC - succinyl-CoA synthetase subunit beta (NCBI), at 762,237 to 763,403 sucC Position (kb) 760 761 762Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 759.888 kb on + strand, within sucAat 759.888 kb on + strand, within sucAat 759.918 kb on + strand, within sucAat 759.959 kb on + strand, within sucAat 759.961 kb on + strand, within sucAat 759.961 kb on + strand, within sucAat 760.247 kb on + strand, within sucAat 760.469 kb on + strandat 760.665 kb on + strandat 760.667 kb on + strandat 760.880 kb on + strandat 760.880 kb on + strand, within sucBat 761.505 kb on + strand, within sucBat 761.728 kb on + strand, within sucBat 761.860 kb on + strandat 761.970 kb on + strandat 761.992 kb on + strandat 762.001 kb on - strandat 762.146 kb on + strandat 762.154 kb on - strandat 762.154 kb on - strandat 762.196 kb on + strandat 762.223 kb on + strandat 762.284 kb on - strandat 762.308 kb on - strandat 762.310 kb on + strandat 762.318 kb on - strandat 762.398 kb on - strand, within sucCat 762.398 kb on - strand, within sucCat 762.600 kb on - strand, within sucCat 762.708 kb on + strand, within sucCat 762.708 kb on + strand, within sucCat 762.713 kb on + strand, within sucC

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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759,888 + sucA b0726 0.70 -1.4
759,888 + sucA b0726 0.70 -0.7
759,918 + sucA b0726 0.71 -0.5
759,959 + sucA b0726 0.72 +1.2
759,961 + sucA b0726 0.73 -0.5
759,961 + sucA b0726 0.73 -0.7
760,247 + sucA b0726 0.83 +0.2
760,469 + -1.9
760,665 + -1.1
760,667 + +0.9
760,880 + +0.7
760,880 + sucB b0727 0.11 -1.3
761,505 + sucB b0727 0.62 -0.9
761,728 + sucB b0727 0.81 -1.4
761,860 + +0.1
761,970 + -0.2
761,992 + +1.9
762,001 - +0.9
762,146 + +0.4
762,154 - -0.4
762,154 - -1.4
762,196 + +0.8
762,223 + -1.4
762,284 - -2.4
762,308 - -3.0
762,310 + -1.1
762,318 - -1.1
762,398 - sucC b0728 0.14 -2.6
762,398 - sucC b0728 0.14 -3.2
762,600 - sucC b0728 0.31 +1.1
762,708 + sucC b0728 0.40 -2.2
762,708 + sucC b0728 0.40 -0.5
762,713 + sucC b0728 0.41 -0.9

Or see this region's nucleotide sequence