Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafC and yafD are separated by 240 nucleotides yafD and yafE are separated by 3 nucleotides yafE and mltD are separated by 47 nucleotides
b0208: yafC - predicted DNA-binding transcriptional regulator (NCBI), at 229,967 to 230,881
yafC
b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922
yafD
b0210: yafE - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 231,926 to 232,549
yafE
b0211: mltD - predicted membrane-bound lytic murein transglycosylase D (NCBI), at 232,597 to 233,955
mltD
Position (kb) 231
232 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 230.208 kb on - strand, within yafC at 230.223 kb on - strand, within yafC at 230.230 kb on + strand, within yafC at 230.244 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.282 kb on + strand, within yafC at 230.282 kb on + strand, within yafC at 230.323 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.572 kb on + strand, within yafC at 230.600 kb on - strand, within yafC at 230.611 kb on + strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 231.017 kb on - strand at 231.145 kb on + strand at 231.161 kb on - strand at 231.161 kb on - strand at 231.183 kb on - strand at 231.235 kb on + strand, within yafD at 231.446 kb on + strand, within yafD at 231.446 kb on + strand, within yafD at 231.535 kb on + strand, within yafD at 231.583 kb on - strand, within yafD at 231.601 kb on + strand at 231.621 kb on + strand, within yafD at 231.627 kb on - strand, within yafD at 231.627 kb on - strand, within yafD at 231.659 kb on + strand, within yafD at 231.659 kb on + strand, within yafD at 231.664 kb on - strand, within yafD at 231.664 kb on - strand, within yafD at 231.667 kb on - strand, within yafD at 231.755 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.837 kb on + strand, within yafD at 231.851 kb on + strand at 231.851 kb on + strand at 231.935 kb on - strand at 231.935 kb on - strand at 231.938 kb on + strand at 231.946 kb on - strand at 231.970 kb on + strand at 231.983 kb on + strand at 231.983 kb on + strand at 232.010 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.303 kb on + strand, within yafE at 232.469 kb on + strand, within yafE at 232.656 kb on + strand at 232.678 kb on - strand at 232.728 kb on - strand at 232.728 kb on - strand at 232.760 kb on - strand, within mltD at 232.830 kb on + strand, within mltD at 232.830 kb on + strand, within mltD at 232.839 kb on + strand, within mltD at 232.839 kb on + strand, within mltD
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 230,208 - yafC b0208 0.26 +1.2 230,223 - yafC b0208 0.28 -0.4 230,230 + yafC b0208 0.29 +1.1 230,244 - yafC b0208 0.30 +0.7 230,276 - yafC b0208 0.34 -1.1 230,276 - yafC b0208 0.34 +4.0 230,282 + yafC b0208 0.34 +1.2 230,282 + yafC b0208 0.34 -0.1 230,323 - yafC b0208 0.39 +0.7 230,549 - yafC b0208 0.64 +1.5 230,549 - yafC b0208 0.64 +0.5 230,572 + yafC b0208 0.66 -0.3 230,600 - yafC b0208 0.69 -0.4 230,611 + yafC b0208 0.70 -1.8 230,732 - yafC b0208 0.84 -0.4 230,732 - yafC b0208 0.84 +0.2 230,732 - yafC b0208 0.84 -2.0 230,785 - yafC b0208 0.89 -0.4 230,785 - yafC b0208 0.89 +2.4 231,017 - +1.4 231,145 + +1.1 231,161 - -0.3 231,161 - -1.7 231,183 - +1.6 231,235 + yafD b0209 0.14 +0.4 231,446 + yafD b0209 0.40 -1.5 231,446 + yafD b0209 0.40 +0.5 231,535 + yafD b0209 0.52 +1.8 231,583 - yafD b0209 0.58 -1.9 231,601 + +0.7 231,621 + yafD b0209 0.62 +0.3 231,627 - yafD b0209 0.63 -1.7 231,627 - yafD b0209 0.63 -1.7 231,659 + yafD b0209 0.67 +0.7 231,659 + yafD b0209 0.67 +0.8 231,664 - yafD b0209 0.68 +1.3 231,664 - yafD b0209 0.68 -2.4 231,667 - yafD b0209 0.68 -0.0 231,755 + yafD b0209 0.79 -0.2 231,828 + yafD b0209 0.88 +1.4 231,828 + yafD b0209 0.88 +0.3 231,837 + yafD b0209 0.89 +0.9 231,851 + -2.0 231,851 + +0.7 231,935 - -0.8 231,935 - -0.2 231,938 + +0.2 231,946 - +1.6 231,970 + -1.5 231,983 + +0.9 231,983 + -1.2 232,010 + yafE b0210 0.13 +0.2 232,254 + yafE b0210 0.53 +2.1 232,254 + yafE b0210 0.53 +2.7 232,254 + yafE b0210 0.53 +0.7 232,303 + yafE b0210 0.60 -2.6 232,469 + yafE b0210 0.87 +1.6 232,656 + +0.6 232,678 - -0.8 232,728 - +1.5 232,728 - -1.0 232,760 - mltD b0211 0.12 +0.7 232,830 + mltD b0211 0.17 -0.3 232,830 + mltD b0211 0.17 +0.4 232,839 + mltD b0211 0.18 -1.5 232,839 + mltD b0211 0.18 +0.7
Or see this region's nucleotide sequence