Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemL and clcA are separated by 224 nucleotides clcA and yadR are separated by 81 nucleotides yadR and yadS are separated by 46 nucleotides
b0154: hemL - glutamate-1-semialdehyde aminotransferase (NCBI), at 173,602 to 174,882
hemL
b0155: clcA - chloride channel protein (NCBI), at 175,107 to 176,528
clcA
b0156: yadR - hypothetical protein (NCBI), at 176,610 to 176,954
yadR
b0157: yadS - conserved inner membrane protein (NCBI), at 177,001 to 177,624
yadS
Position (kb) 175
176
177 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 174.690 kb on - strand, within hemL at 174.958 kb on + strand at 174.968 kb on + strand at 174.968 kb on + strand at 174.993 kb on - strand at 174.993 kb on - strand at 175.096 kb on - strand at 175.167 kb on - strand at 175.227 kb on + strand at 175.273 kb on - strand, within clcA at 175.273 kb on - strand, within clcA at 175.273 kb on - strand, within clcA at 175.284 kb on + strand, within clcA at 175.308 kb on + strand, within clcA at 175.316 kb on - strand, within clcA at 175.316 kb on - strand, within clcA at 175.345 kb on - strand, within clcA at 175.345 kb on - strand, within clcA at 175.483 kb on - strand, within clcA at 175.491 kb on - strand, within clcA at 175.531 kb on - strand, within clcA at 175.536 kb on + strand, within clcA at 175.536 kb on + strand, within clcA at 175.536 kb on + strand, within clcA at 175.569 kb on + strand, within clcA at 175.569 kb on + strand, within clcA at 175.579 kb on - strand, within clcA at 175.582 kb on + strand, within clcA at 175.583 kb on + strand, within clcA at 175.607 kb on + strand, within clcA at 175.627 kb on + strand, within clcA at 175.635 kb on - strand, within clcA at 175.648 kb on + strand, within clcA at 175.648 kb on + strand, within clcA at 175.758 kb on + strand, within clcA at 175.762 kb on + strand, within clcA at 175.781 kb on + strand, within clcA at 175.818 kb on - strand, within clcA at 175.850 kb on + strand, within clcA at 175.851 kb on + strand, within clcA at 175.851 kb on + strand, within clcA at 175.864 kb on + strand, within clcA at 175.933 kb on - strand, within clcA at 175.933 kb on - strand, within clcA at 175.949 kb on - strand, within clcA at 175.949 kb on - strand, within clcA at 175.959 kb on + strand, within clcA at 176.017 kb on - strand, within clcA at 176.017 kb on - strand, within clcA at 176.136 kb on + strand, within clcA at 176.173 kb on + strand, within clcA at 176.210 kb on - strand, within clcA at 176.210 kb on - strand, within clcA at 176.295 kb on + strand, within clcA at 176.356 kb on + strand, within clcA at 176.441 kb on - strand at 176.503 kb on - strand at 176.503 kb on - strand at 176.514 kb on + strand at 176.540 kb on + strand at 177.002 kb on - strand at 177.019 kb on - strand at 177.019 kb on - strand at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.084 kb on - strand, within yadS at 177.221 kb on - strand, within yadS at 177.257 kb on + strand, within yadS at 177.300 kb on - strand, within yadS at 177.300 kb on - strand, within yadS at 177.333 kb on + strand, within yadS at 177.341 kb on - strand, within yadS
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 174,690 - hemL b0154 0.85 +2.6 174,958 + -2.4 174,968 + +1.6 174,968 + -0.2 174,993 - +1.9 174,993 - +1.5 175,096 - -0.2 175,167 - +0.6 175,227 + +2.1 175,273 - clcA b0155 0.12 +0.4 175,273 - clcA b0155 0.12 -1.7 175,273 - clcA b0155 0.12 -0.6 175,284 + clcA b0155 0.12 +0.8 175,308 + clcA b0155 0.14 +0.8 175,316 - clcA b0155 0.15 +0.7 175,316 - clcA b0155 0.15 +1.6 175,345 - clcA b0155 0.17 +1.9 175,345 - clcA b0155 0.17 +2.6 175,483 - clcA b0155 0.26 -2.0 175,491 - clcA b0155 0.27 +0.9 175,531 - clcA b0155 0.30 +2.2 175,536 + clcA b0155 0.30 -1.6 175,536 + clcA b0155 0.30 -0.2 175,536 + clcA b0155 0.30 -0.9 175,569 + clcA b0155 0.32 +0.5 175,569 + clcA b0155 0.32 -0.9 175,579 - clcA b0155 0.33 +1.3 175,582 + clcA b0155 0.33 -1.0 175,583 + clcA b0155 0.33 +1.0 175,607 + clcA b0155 0.35 +1.3 175,627 + clcA b0155 0.37 -1.6 175,635 - clcA b0155 0.37 +1.5 175,648 + clcA b0155 0.38 +1.4 175,648 + clcA b0155 0.38 +1.5 175,758 + clcA b0155 0.46 -1.5 175,762 + clcA b0155 0.46 +0.3 175,781 + clcA b0155 0.47 -1.9 175,818 - clcA b0155 0.50 -0.0 175,850 + clcA b0155 0.52 +2.0 175,851 + clcA b0155 0.52 -0.1 175,851 + clcA b0155 0.52 -0.9 175,864 + clcA b0155 0.53 +2.5 175,933 - clcA b0155 0.58 -1.0 175,933 - clcA b0155 0.58 +0.7 175,949 - clcA b0155 0.59 -1.4 175,949 - clcA b0155 0.59 +1.1 175,959 + clcA b0155 0.60 +0.8 176,017 - clcA b0155 0.64 +1.5 176,017 - clcA b0155 0.64 -0.2 176,136 + clcA b0155 0.72 -3.5 176,173 + clcA b0155 0.75 +1.2 176,210 - clcA b0155 0.78 +1.0 176,210 - clcA b0155 0.78 +0.7 176,295 + clcA b0155 0.84 -1.9 176,356 + clcA b0155 0.88 -0.4 176,441 - -2.5 176,503 - -2.2 176,503 - -0.3 176,514 + +2.3 176,540 + -1.5 177,002 - -0.2 177,019 - -0.7 177,019 - +1.7 177,084 - yadS b0157 0.13 -0.7 177,084 - yadS b0157 0.13 +0.8 177,084 - yadS b0157 0.13 +0.9 177,084 - yadS b0157 0.13 -0.3 177,084 - yadS b0157 0.13 +1.7 177,084 - yadS b0157 0.13 -1.3 177,221 - yadS b0157 0.35 -2.0 177,257 + yadS b0157 0.41 +2.4 177,300 - yadS b0157 0.48 -0.6 177,300 - yadS b0157 0.48 -0.1 177,333 + yadS b0157 0.53 -1.7 177,341 - yadS b0157 0.54 +0.2
Or see this region's nucleotide sequence