Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF975 and GFF976 are separated by 2 nucleotides GFF976 and GFF977 are separated by 181 nucleotides GFF977 and GFF978 overlap by 4 nucleotides GFF978 and GFF979 are separated by 397 nucleotides
GFF975 - FIG074102: hypothetical protein, at 161,806 to 162,717
GFF975
GFF976 - hypothetical protein, at 162,720 to 162,833
GFF976
GFF977 - Putative inner membrane or exported, at 163,015 to 163,383
GFF977
GFF978 - Dienelactone hydrolase family, at 163,380 to 164,054
GFF978
GFF979 - Uxu operon transcriptional regulator, at 164,452 to 165,177
GFF979
Position (kb)
163
164
165 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 162.450 kb on - strand, within GFF975 at 162.718 kb on + strand at 162.718 kb on + strand at 162.847 kb on - strand at 162.871 kb on + strand at 162.968 kb on + strand at 163.043 kb on - strand at 163.484 kb on + strand, within GFF978 at 163.517 kb on - strand, within GFF978 at 163.524 kb on - strand, within GFF978 at 163.588 kb on + strand, within GFF978 at 163.698 kb on + strand, within GFF978 at 163.724 kb on + strand, within GFF978 at 163.724 kb on + strand, within GFF978 at 163.724 kb on + strand, within GFF978 at 163.725 kb on - strand, within GFF978 at 163.725 kb on - strand, within GFF978 at 163.725 kb on - strand, within GFF978 at 163.732 kb on - strand, within GFF978 at 163.773 kb on + strand, within GFF978 at 163.773 kb on + strand, within GFF978 at 163.883 kb on + strand, within GFF978 at 163.884 kb on - strand, within GFF978 at 163.885 kb on + strand, within GFF978 at 163.885 kb on + strand, within GFF978 at 163.885 kb on + strand, within GFF978 at 163.886 kb on - strand, within GFF978 at 163.886 kb on - strand, within GFF978 at 163.886 kb on - strand, within GFF978 at 163.886 kb on - strand, within GFF978 at 163.940 kb on + strand, within GFF978 at 164.049 kb on + strand at 164.049 kb on + strand at 164.050 kb on - strand at 164.104 kb on - strand at 164.118 kb on - strand at 164.351 kb on + strand at 164.361 kb on - strand at 164.361 kb on - strand at 164.361 kb on - strand at 164.545 kb on - strand, within GFF979 at 164.618 kb on - strand, within GFF979 at 164.674 kb on - strand, within GFF979 at 164.773 kb on - strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.890 kb on - strand, within GFF979 at 164.890 kb on - strand, within GFF979 at 164.958 kb on + strand, within GFF979
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 162,450 - GFF975 0.71 +0.8 162,718 + -0.7 162,718 + +1.0 162,847 - -0.2 162,871 + +0.3 162,968 + -0.6 163,043 - +0.1 163,484 + GFF978 0.15 +0.2 163,517 - GFF978 0.20 -0.7 163,524 - GFF978 0.21 -0.2 163,588 + GFF978 0.31 +0.5 163,698 + GFF978 0.47 -0.2 163,724 + GFF978 0.51 +0.5 163,724 + GFF978 0.51 -0.0 163,724 + GFF978 0.51 -0.0 163,725 - GFF978 0.51 -0.2 163,725 - GFF978 0.51 +0.2 163,725 - GFF978 0.51 -0.5 163,732 - GFF978 0.52 -2.5 163,773 + GFF978 0.58 -1.3 163,773 + GFF978 0.58 -0.6 163,883 + GFF978 0.75 -1.1 163,884 - GFF978 0.75 -0.4 163,885 + GFF978 0.75 -0.0 163,885 + GFF978 0.75 +1.0 163,885 + GFF978 0.75 -0.0 163,886 - GFF978 0.75 -0.8 163,886 - GFF978 0.75 -0.1 163,886 - GFF978 0.75 +0.0 163,886 - GFF978 0.75 -0.6 163,940 + GFF978 0.83 -2.2 164,049 + +0.1 164,049 + -0.1 164,050 - +0.0 164,104 - -0.7 164,118 - +0.6 164,351 + -3.1 164,361 - -0.3 164,361 - -0.9 164,361 - -0.0 164,545 - GFF979 0.13 -1.0 164,618 - GFF979 0.23 -0.9 164,674 - GFF979 0.31 -0.4 164,773 - GFF979 0.44 -1.5 164,889 + GFF979 0.60 -0.9 164,889 + GFF979 0.60 +0.5 164,889 + GFF979 0.60 +0.2 164,890 - GFF979 0.60 -0.3 164,890 - GFF979 0.60 +0.0 164,958 + GFF979 0.70 +0.1
Or see this region's nucleotide sequence