Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF672 and GFF673 overlap by 17 nucleotides GFF673 and GFF674 overlap by 8 nucleotides
GFF672 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 28,271 to 29,428
GFF672
GFF673 - Biotin synthesis protein BioC, at 29,412 to 30,167
GFF673
GFF674 - Dethiobiotin synthetase (EC 6.3.3.3), at 30,160 to 30,846
GFF674
Position (kb) 29
30
31 Strain fitness (log2 ratio) -1
0
1 at 28.425 kb on + strand, within GFF672 at 28.585 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.802 kb on + strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 28.803 kb on - strand, within GFF672 at 29.426 kb on + strand at 29.426 kb on + strand at 29.577 kb on - strand, within GFF673 at 29.577 kb on - strand, within GFF673 at 29.577 kb on - strand, within GFF673 at 29.790 kb on + strand, within GFF673 at 29.791 kb on - strand, within GFF673 at 30.080 kb on + strand, within GFF673 at 30.197 kb on - strand at 30.249 kb on - strand, within GFF674 at 30.249 kb on - strand, within GFF674 at 30.264 kb on - strand, within GFF674 at 30.264 kb on - strand, within GFF674 at 30.268 kb on + strand, within GFF674 at 30.268 kb on + strand, within GFF674 at 30.271 kb on - strand, within GFF674 at 30.384 kb on + strand, within GFF674 at 30.399 kb on + strand, within GFF674 at 30.399 kb on + strand, within GFF674 at 30.525 kb on + strand, within GFF674 at 30.615 kb on + strand, within GFF674 at 30.615 kb on + strand, within GFF674 at 30.616 kb on - strand, within GFF674 at 30.618 kb on + strand, within GFF674 at 30.692 kb on + strand, within GFF674 at 30.693 kb on - strand, within GFF674 at 30.693 kb on - strand, within GFF674 at 30.815 kb on - strand at 30.826 kb on - strand at 31.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 28,425 + GFF672 0.13 +0.3 28,585 + GFF672 0.27 +0.1 28,802 + GFF672 0.46 +0.4 28,802 + GFF672 0.46 +0.5 28,802 + GFF672 0.46 +1.5 28,802 + GFF672 0.46 +0.2 28,803 - GFF672 0.46 -0.5 28,803 - GFF672 0.46 -0.7 28,803 - GFF672 0.46 +1.3 29,426 + -0.4 29,426 + +0.4 29,577 - GFF673 0.22 +0.2 29,577 - GFF673 0.22 -0.0 29,577 - GFF673 0.22 +0.0 29,790 + GFF673 0.50 +0.4 29,791 - GFF673 0.50 -0.1 30,080 + GFF673 0.88 +0.5 30,197 - -1.0 30,249 - GFF674 0.13 +1.2 30,249 - GFF674 0.13 +0.3 30,264 - GFF674 0.15 -0.3 30,264 - GFF674 0.15 -0.7 30,268 + GFF674 0.16 -0.4 30,268 + GFF674 0.16 +0.1 30,271 - GFF674 0.16 -1.3 30,384 + GFF674 0.33 -0.7 30,399 + GFF674 0.35 -0.5 30,399 + GFF674 0.35 -0.7 30,525 + GFF674 0.53 -0.6 30,615 + GFF674 0.66 -0.2 30,615 + GFF674 0.66 +0.0 30,616 - GFF674 0.66 +1.0 30,618 + GFF674 0.67 +0.3 30,692 + GFF674 0.77 -1.3 30,693 - GFF674 0.78 -0.0 30,693 - GFF674 0.78 -0.7 30,815 - +0.1 30,826 - -1.3 31,109 - -0.7
Or see this region's nucleotide sequence