Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4723

Experiment: phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4721 and GFF4722 overlap by 10 nucleotidesGFF4722 and GFF4723 overlap by 1 nucleotidesGFF4723 and GFF4724 overlap by 4 nucleotides GFF4721 - [NiFe] hydrogenase nickel incorporation-associated protein HypB, at 30,003 to 30,875 GFF4721 GFF4722 - [NiFe] hydrogenase metallocenter assembly protein HypC, at 30,866 to 31,138 GFF4722 GFF4723 - [NiFe] hydrogenase metallocenter assembly protein HypD, at 31,138 to 32,259 GFF4723 GFF4724 - [NiFe] hydrogenase metallocenter assembly protein HypE, at 32,256 to 33,266 GFF4724 Position (kb) 31 32 33Strain fitness (log2 ratio) -3 -2 -1 0 1at 30.266 kb on + strand, within GFF4721at 30.398 kb on - strand, within GFF4721at 30.627 kb on - strand, within GFF4721at 31.084 kb on + strand, within GFF4722at 31.136 kb on + strandat 31.137 kb on - strandat 31.157 kb on - strandat 31.305 kb on - strand, within GFF4723at 31.497 kb on + strand, within GFF4723at 31.691 kb on + strand, within GFF4723at 31.833 kb on - strand, within GFF4723at 31.833 kb on - strand, within GFF4723at 31.897 kb on + strand, within GFF4723at 32.083 kb on + strand, within GFF4723at 32.084 kb on - strand, within GFF4723at 32.084 kb on - strand, within GFF4723at 32.266 kb on + strandat 32.267 kb on - strandat 32.267 kb on - strandat 32.324 kb on + strandat 32.433 kb on + strand, within GFF4724at 32.433 kb on + strand, within GFF4724at 32.434 kb on - strand, within GFF4724at 32.597 kb on + strand, within GFF4724at 32.683 kb on + strand, within GFF4724at 32.688 kb on - strand, within GFF4724at 32.777 kb on + strand, within GFF4724at 32.864 kb on - strand, within GFF4724at 32.892 kb on - strand, within GFF4724at 33.207 kb on + strandat 33.250 kb on + strandat 33.257 kb on + strandat 33.259 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI
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30,266 + GFF4721 0.30 -0.1
30,398 - GFF4721 0.45 +1.0
30,627 - GFF4721 0.71 +0.2
31,084 + GFF4722 0.80 +0.7
31,136 + -0.2
31,137 - -1.5
31,157 - -1.0
31,305 - GFF4723 0.15 -0.3
31,497 + GFF4723 0.32 +0.1
31,691 + GFF4723 0.49 -1.1
31,833 - GFF4723 0.62 -1.0
31,833 - GFF4723 0.62 -1.0
31,897 + GFF4723 0.68 -3.4
32,083 + GFF4723 0.84 -0.3
32,084 - GFF4723 0.84 +0.4
32,084 - GFF4723 0.84 +0.0
32,266 + -0.1
32,267 - -1.3
32,267 - +0.2
32,324 + +0.4
32,433 + GFF4724 0.18 +0.1
32,433 + GFF4724 0.18 -0.1
32,434 - GFF4724 0.18 +0.5
32,597 + GFF4724 0.34 -0.3
32,683 + GFF4724 0.42 +0.6
32,688 - GFF4724 0.43 +0.1
32,777 + GFF4724 0.52 -0.1
32,864 - GFF4724 0.60 -0.9
32,892 - GFF4724 0.63 -0.4
33,207 + -0.7
33,250 + +0.2
33,257 + -1.1
33,259 + +0.3

Or see this region's nucleotide sequence