Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4460 and GFF4461 overlap by 8 nucleotides GFF4461 and GFF4462 overlap by 4 nucleotides GFF4462 and GFF4658 are separated by 348 nucleotides GFF4658 and GFF4463 are separated by 163 nucleotides GFF4463 and GFF4464 are separated by 238 nucleotides
GFF4460 - Putative inner membrane protein, at 245,018 to 246,658
GFF4460
GFF4461 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 246,651 to 247,577
GFF4461
GFF4462 - Bactoprenol-linked glucose translocase, at 247,574 to 247,849
GFF4462
GFF4658 - tRNA-Pseudo-TCT, at 248,198 to 248,270
GFF4658
GFF4463 - copE1, at 248,434 to 248,565
GFF4463
GFF4464 - sensor histidine kinase, at 248,804 to 249,133
GFF4464
Position (kb) 247
248 Strain fitness (log2 ratio) -1
0
1 at 247.184 kb on + strand, within GFF4461 at 247.184 kb on + strand, within GFF4461 at 247.184 kb on + strand, within GFF4461 at 247.294 kb on - strand, within GFF4461 at 247.728 kb on + strand, within GFF4462 at 247.729 kb on - strand, within GFF4462 at 247.843 kb on + strand at 248.043 kb on - strand at 248.348 kb on + strand at 248.349 kb on - strand at 248.349 kb on - strand at 248.361 kb on - strand at 248.661 kb on - strand at 248.661 kb on - strand at 248.667 kb on - strand at 248.842 kb on + strand, within GFF4464
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 247,184 + GFF4461 0.57 +0.3 247,184 + GFF4461 0.57 +0.3 247,184 + GFF4461 0.57 -0.1 247,294 - GFF4461 0.69 -0.4 247,728 + GFF4462 0.56 -0.5 247,729 - GFF4462 0.56 -0.6 247,843 + -0.6 248,043 - -0.7 248,348 + +1.2 248,349 - -0.2 248,349 - +0.3 248,361 - +0.3 248,661 - +0.4 248,661 - +0.4 248,667 - -0.4 248,842 + GFF4464 0.12 +0.3
Or see this region's nucleotide sequence