Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4452 and GFF4453 are separated by 9 nucleotides GFF4453 and GFF4454 are separated by 45 nucleotides GFF4454 and GFF4455 are separated by 603 nucleotides
GFF4452 - Fimbriae-like adhesin SfmH, at 238,889 to 239,896
GFF4452
GFF4453 - Fimbriae-like periplasmic protein SfmF, at 239,906 to 240,424
GFF4453
GFF4454 - Transcriptional regulator of fimbriae expression FimZ (LuxR/UhpA family), at 240,470 to 241,102
GFF4454
GFF4455 - Transcriptional regulator of fimbriae expression FimY, at 241,706 to 242,428
GFF4455
Position (kb) 240
241
242 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 239.541 kb on + strand, within GFF4452 at 239.605 kb on + strand, within GFF4452 at 239.614 kb on + strand, within GFF4452 at 239.720 kb on - strand, within GFF4452 at 239.722 kb on + strand, within GFF4452 at 239.806 kb on + strand at 240.167 kb on - strand, within GFF4453 at 240.167 kb on - strand, within GFF4453 at 240.351 kb on - strand, within GFF4453 at 240.353 kb on - strand, within GFF4453 at 240.422 kb on + strand at 240.423 kb on - strand at 240.471 kb on + strand at 240.539 kb on + strand, within GFF4454 at 241.063 kb on + strand at 241.108 kb on + strand at 241.789 kb on + strand, within GFF4455 at 242.021 kb on - strand, within GFF4455 at 242.088 kb on - strand, within GFF4455
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 239,541 + GFF4452 0.65 +1.1 239,605 + GFF4452 0.71 -3.0 239,614 + GFF4452 0.72 +0.5 239,720 - GFF4452 0.82 -1.0 239,722 + GFF4452 0.83 +0.4 239,806 + +0.9 240,167 - GFF4453 0.50 -0.4 240,167 - GFF4453 0.50 -1.3 240,351 - GFF4453 0.86 -0.9 240,353 - GFF4453 0.86 -0.7 240,422 + -0.6 240,423 - +0.4 240,471 + +0.1 240,539 + GFF4454 0.11 -1.5 241,063 + +0.5 241,108 + -0.7 241,789 + GFF4455 0.11 -0.7 242,021 - GFF4455 0.44 -0.7 242,088 - GFF4455 0.53 +1.9
Or see this region's nucleotide sequence