Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF38

Experiment: phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF37 and GFF38 are separated by 12 nucleotidesGFF38 and GFF39 are separated by 71 nucleotidesGFF39 and GFF40 overlap by 4 nucleotides GFF37 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 15,087 to 16,046 GFF37 GFF38 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 16,059 to 19,493 GFF38 GFF39 - Ribonuclease HII (EC 3.1.26.4), at 19,565 to 20,161 GFF39 GFF40 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 20,158 to 21,306 GFF40 Position (kb) 16 17 18 19 20Strain fitness (log2 ratio) -2 -1 0 1 2at 15.100 kb on + strandat 19.754 kb on - strand, within GFF39at 19.865 kb on - strand, within GFF39

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Per-strain Table

Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI
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15,100 + -2.0
19,754 - GFF39 0.32 -0.0
19,865 - GFF39 0.50 +1.9

Or see this region's nucleotide sequence