Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3602 and GFF3603 are separated by 57 nucleotides GFF3603 and GFF3604 overlap by 16 nucleotides GFF3604 and GFF3605 overlap by 11 nucleotides
GFF3602 - Type III secretion cytoplasmic protein (YscL), at 354,306 to 355,298
GFF3602
GFF3603 - Secretion system apparatus protein SsaM, at 355,356 to 355,724
GFF3603
GFF3604 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 355,709 to 357,754
GFF3604
GFF3605 - Type III secretion cytoplasmic ATP synthase (EC 3.6.3.14, YscN,SpaL,MxiB,HrcN,EscN), at 357,744 to 359,045
GFF3605
Position (kb) 355
356
357
358 Strain fitness (log2 ratio) -2
-1
0
1 at 354.792 kb on - strand, within GFF3602 at 354.850 kb on + strand, within GFF3602 at 354.906 kb on - strand, within GFF3602 at 354.986 kb on - strand, within GFF3602 at 355.203 kb on - strand at 355.471 kb on - strand, within GFF3603 at 355.669 kb on + strand, within GFF3603 at 355.778 kb on - strand at 355.962 kb on + strand, within GFF3604 at 356.056 kb on - strand, within GFF3604 at 356.086 kb on - strand, within GFF3604 at 356.386 kb on - strand, within GFF3604 at 356.425 kb on + strand, within GFF3604 at 356.498 kb on - strand, within GFF3604 at 356.586 kb on + strand, within GFF3604 at 356.790 kb on + strand, within GFF3604 at 356.791 kb on - strand, within GFF3604 at 356.939 kb on - strand, within GFF3604 at 357.123 kb on + strand, within GFF3604 at 357.296 kb on - strand, within GFF3604 at 357.374 kb on + strand, within GFF3604 at 357.510 kb on - strand, within GFF3604 at 357.510 kb on - strand, within GFF3604 at 357.532 kb on + strand, within GFF3604 at 357.554 kb on - strand at 357.799 kb on - strand at 357.841 kb on + strand at 357.863 kb on - strand at 357.884 kb on + strand, within GFF3605 at 357.886 kb on + strand, within GFF3605 at 357.944 kb on - strand, within GFF3605 at 357.998 kb on - strand, within GFF3605 at 358.047 kb on - strand, within GFF3605 at 358.179 kb on - strand, within GFF3605 at 358.271 kb on + strand, within GFF3605 at 358.271 kb on + strand, within GFF3605 at 358.512 kb on + strand, within GFF3605 at 358.610 kb on - strand, within GFF3605 at 358.616 kb on + strand, within GFF3605 at 358.640 kb on + strand, within GFF3605 at 358.644 kb on - strand, within GFF3605 at 358.653 kb on + strand, within GFF3605 at 358.653 kb on + strand, within GFF3605 at 358.733 kb on - strand, within GFF3605
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 354,792 - GFF3602 0.49 -2.3 354,850 + GFF3602 0.55 -0.6 354,906 - GFF3602 0.60 -1.1 354,986 - GFF3602 0.68 +0.3 355,203 - -0.5 355,471 - GFF3603 0.31 -0.5 355,669 + GFF3603 0.85 +0.6 355,778 - -2.0 355,962 + GFF3604 0.12 -0.2 356,056 - GFF3604 0.17 -0.4 356,086 - GFF3604 0.18 -0.7 356,386 - GFF3604 0.33 -0.6 356,425 + GFF3604 0.35 -0.5 356,498 - GFF3604 0.39 -0.2 356,586 + GFF3604 0.43 -1.0 356,790 + GFF3604 0.53 +0.0 356,791 - GFF3604 0.53 +0.2 356,939 - GFF3604 0.60 -0.5 357,123 + GFF3604 0.69 -0.3 357,296 - GFF3604 0.78 -0.8 357,374 + GFF3604 0.81 +0.9 357,510 - GFF3604 0.88 -0.0 357,510 - GFF3604 0.88 -0.3 357,532 + GFF3604 0.89 +0.0 357,554 - -0.7 357,799 - +1.5 357,841 + +0.8 357,863 - -0.1 357,884 + GFF3605 0.11 -0.8 357,886 + GFF3605 0.11 +0.3 357,944 - GFF3605 0.15 -0.9 357,998 - GFF3605 0.20 +1.3 358,047 - GFF3605 0.23 -1.8 358,179 - GFF3605 0.33 +0.5 358,271 + GFF3605 0.40 +0.6 358,271 + GFF3605 0.40 +0.1 358,512 + GFF3605 0.59 -0.3 358,610 - GFF3605 0.67 +0.3 358,616 + GFF3605 0.67 +0.4 358,640 + GFF3605 0.69 +0.4 358,644 - GFF3605 0.69 -0.9 358,653 + GFF3605 0.70 -0.6 358,653 + GFF3605 0.70 +0.1 358,733 - GFF3605 0.76 -1.9
Or see this region's nucleotide sequence