Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3512 and GFF3513 are separated by 148 nucleotides GFF3513 and GFF3514 are separated by 42 nucleotides GFF3514 and GFF3515 are separated by 106 nucleotides GFF3515 and GFF3516 are separated by 96 nucleotides
GFF3512 - 2-deoxyglucose-6-phosphate hydrolase YniC, at 264,393 to 265,061
GFF3512
GFF3513 - Putative membrane protein, at 265,210 to 265,746
GFF3513
GFF3514 - Ribulosamine/erythrulosamine 3-kinase potentially involved in protein deglycation, at 265,789 to 266,649
GFF3514
GFF3515 - FIG00637864: hypothetical protein, at 266,756 to 267,046
GFF3515
GFF3516 - 6-phosphofructokinase class II (EC 2.7.1.11), at 267,143 to 268,075
GFF3516
Position (kb)
265
266
267 Strain fitness (log2 ratio)
-1
0
1 at 264.825 kb on - strand, within GFF3512 at 264.945 kb on - strand, within GFF3512 at 264.949 kb on - strand, within GFF3512 at 264.949 kb on - strand, within GFF3512 at 264.955 kb on - strand, within GFF3512 at 265.127 kb on - strand at 265.128 kb on + strand at 265.129 kb on - strand at 265.129 kb on - strand at 265.216 kb on + strand at 265.217 kb on - strand at 265.318 kb on + strand, within GFF3513 at 265.318 kb on + strand, within GFF3513 at 265.318 kb on + strand, within GFF3513 at 265.376 kb on + strand, within GFF3513 at 265.377 kb on - strand, within GFF3513 at 265.625 kb on + strand, within GFF3513 at 265.636 kb on - strand, within GFF3513 at 265.791 kb on - strand at 265.883 kb on + strand, within GFF3514 at 265.943 kb on + strand, within GFF3514 at 266.029 kb on - strand, within GFF3514 at 266.030 kb on + strand, within GFF3514 at 266.030 kb on + strand, within GFF3514 at 266.157 kb on + strand, within GFF3514 at 266.157 kb on + strand, within GFF3514 at 266.158 kb on - strand, within GFF3514 at 266.546 kb on - strand, within GFF3514 at 266.649 kb on + strand at 266.712 kb on - strand at 266.718 kb on + strand at 266.812 kb on + strand, within GFF3515 at 266.812 kb on + strand, within GFF3515 at 267.077 kb on + strand at 267.166 kb on - strand at 267.202 kb on + strand at 267.305 kb on - strand, within GFF3516 at 267.378 kb on - strand, within GFF3516 at 267.440 kb on + strand, within GFF3516 at 267.577 kb on + strand, within GFF3516
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 264,825 - GFF3512 0.65 +0.9 264,945 - GFF3512 0.83 -0.0 264,949 - GFF3512 0.83 +0.5 264,949 - GFF3512 0.83 -0.7 264,955 - GFF3512 0.84 -0.2 265,127 - -1.3 265,128 + +0.7 265,129 - +0.4 265,129 - -0.3 265,216 + -0.4 265,217 - -0.1 265,318 + GFF3513 0.20 -0.5 265,318 + GFF3513 0.20 -0.3 265,318 + GFF3513 0.20 -0.2 265,376 + GFF3513 0.31 -0.1 265,377 - GFF3513 0.31 -1.1 265,625 + GFF3513 0.77 +0.3 265,636 - GFF3513 0.79 -0.6 265,791 - +0.6 265,883 + GFF3514 0.11 -0.6 265,943 + GFF3514 0.18 +0.1 266,029 - GFF3514 0.28 -0.7 266,030 + GFF3514 0.28 -0.2 266,030 + GFF3514 0.28 -0.9 266,157 + GFF3514 0.43 -0.4 266,157 + GFF3514 0.43 -1.6 266,158 - GFF3514 0.43 -0.7 266,546 - GFF3514 0.88 +0.6 266,649 + +0.7 266,712 - +0.1 266,718 + +0.5 266,812 + GFF3515 0.19 +0.7 266,812 + GFF3515 0.19 -0.5 267,077 + -0.9 267,166 - -1.7 267,202 + +0.5 267,305 - GFF3516 0.17 -0.0 267,378 - GFF3516 0.25 +1.0 267,440 + GFF3516 0.32 -0.8 267,577 + GFF3516 0.47 -0.4
Or see this region's nucleotide sequence