Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3179 and GFF3180 are separated by 234 nucleotides GFF3180 and GFF3181 overlap by 4 nucleotides GFF3181 and GFF3182 are separated by 387 nucleotides
GFF3179 - iron-chelator utilization protein, at 184,294 to 185,061
GFF3179
GFF3180 - Transcriptional regulator, PadR family, at 185,296 to 185,940
GFF3180
GFF3181 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 185,937 to 187,502
GFF3181
GFF3182 - Aerotaxis sensor receptor protein, at 187,890 to 189,410
GFF3182
Position (kb) 185
186
187
188 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 184.975 kb on - strand, within GFF3179 at 184.977 kb on + strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 185.044 kb on + strand at 185.337 kb on + strand at 185.552 kb on - strand, within GFF3180 at 185.652 kb on + strand, within GFF3180 at 185.823 kb on + strand, within GFF3180 at 185.997 kb on + strand at 186.108 kb on - strand, within GFF3181 at 186.108 kb on - strand, within GFF3181 at 186.150 kb on + strand, within GFF3181 at 186.189 kb on - strand, within GFF3181 at 186.549 kb on - strand, within GFF3181 at 186.762 kb on + strand, within GFF3181 at 186.763 kb on - strand, within GFF3181 at 186.794 kb on + strand, within GFF3181 at 186.876 kb on - strand, within GFF3181 at 186.877 kb on + strand, within GFF3181 at 186.877 kb on + strand, within GFF3181 at 186.878 kb on - strand, within GFF3181 at 186.878 kb on - strand, within GFF3181 at 186.879 kb on - strand, within GFF3181 at 186.927 kb on + strand, within GFF3181 at 186.927 kb on + strand, within GFF3181 at 186.948 kb on - strand, within GFF3181 at 187.025 kb on + strand, within GFF3181 at 187.097 kb on + strand, within GFF3181 at 187.221 kb on - strand, within GFF3181 at 187.230 kb on + strand, within GFF3181 at 187.370 kb on + strand at 187.371 kb on - strand at 187.661 kb on - strand at 187.663 kb on + strand at 187.794 kb on + strand at 187.875 kb on + strand at 187.875 kb on + strand at 187.885 kb on + strand at 188.066 kb on + strand, within GFF3182 at 188.103 kb on + strand, within GFF3182 at 188.337 kb on - strand, within GFF3182 at 188.337 kb on - strand, within GFF3182 at 188.341 kb on - strand, within GFF3182 at 188.369 kb on + strand, within GFF3182
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 184,975 - GFF3179 0.89 -0.9 184,977 + GFF3179 0.89 -0.7 184,978 - GFF3179 0.89 +0.3 184,978 - GFF3179 0.89 +0.5 184,978 - GFF3179 0.89 -0.0 185,044 + +0.1 185,337 + -0.2 185,552 - GFF3180 0.40 -1.3 185,652 + GFF3180 0.55 +0.1 185,823 + GFF3180 0.82 +0.1 185,997 + -0.8 186,108 - GFF3181 0.11 -1.3 186,108 - GFF3181 0.11 +0.4 186,150 + GFF3181 0.14 -0.5 186,189 - GFF3181 0.16 +0.2 186,549 - GFF3181 0.39 -0.7 186,762 + GFF3181 0.53 +0.5 186,763 - GFF3181 0.53 +0.6 186,794 + GFF3181 0.55 -0.1 186,876 - GFF3181 0.60 -0.3 186,877 + GFF3181 0.60 +0.0 186,877 + GFF3181 0.60 -0.4 186,878 - GFF3181 0.60 -3.4 186,878 - GFF3181 0.60 +0.5 186,879 - GFF3181 0.60 -1.8 186,927 + GFF3181 0.63 -1.6 186,927 + GFF3181 0.63 -0.1 186,948 - GFF3181 0.65 +0.5 187,025 + GFF3181 0.69 +0.4 187,097 + GFF3181 0.74 -0.2 187,221 - GFF3181 0.82 +0.6 187,230 + GFF3181 0.83 -0.4 187,370 + -0.7 187,371 - -0.0 187,661 - -0.4 187,663 + +0.3 187,794 + +0.5 187,875 + +0.7 187,875 + +0.6 187,885 + -0.9 188,066 + GFF3182 0.12 +0.0 188,103 + GFF3182 0.14 -0.4 188,337 - GFF3182 0.29 +0.7 188,337 - GFF3182 0.29 +0.0 188,341 - GFF3182 0.30 -0.4 188,369 + GFF3182 0.31 +2.0
Or see this region's nucleotide sequence