Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3067 and GFF3068 are separated by 64 nucleotides GFF3068 and GFF3069 are separated by 26 nucleotides GFF3069 and GFF3070 are separated by 61 nucleotides
GFF3067 - Membrane-bound lytic murein transglycosylase C precursor (EC 3.2.1.-), at 75,288 to 76,373
GFF3067
GFF3068 - hypothetical protein, at 76,438 to 76,563
GFF3068
GFF3069 - Nucleoside permease NupG, at 76,590 to 77,846
GFF3069
GFF3070 - Ornithine decarboxylase (EC 4.1.1.17), at 77,908 to 80,043
GFF3070
Position (kb)
76
77
78 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 75.600 kb on - strand, within GFF3067 at 75.605 kb on + strand, within GFF3067 at 75.606 kb on - strand, within GFF3067 at 75.693 kb on + strand, within GFF3067 at 75.809 kb on + strand, within GFF3067 at 75.852 kb on - strand, within GFF3067 at 75.867 kb on + strand, within GFF3067 at 75.868 kb on - strand, within GFF3067 at 75.897 kb on - strand, within GFF3067 at 75.900 kb on + strand, within GFF3067 at 76.000 kb on + strand, within GFF3067 at 76.001 kb on - strand, within GFF3067 at 76.044 kb on - strand, within GFF3067 at 76.110 kb on + strand, within GFF3067 at 76.127 kb on + strand, within GFF3067 at 76.128 kb on - strand, within GFF3067 at 76.210 kb on + strand, within GFF3067 at 76.210 kb on + strand, within GFF3067 at 76.210 kb on + strand, within GFF3067 at 76.211 kb on - strand, within GFF3067 at 76.291 kb on + strand at 76.291 kb on + strand at 76.332 kb on + strand at 76.332 kb on + strand at 76.332 kb on + strand at 76.332 kb on + strand at 76.333 kb on - strand at 76.334 kb on + strand at 76.611 kb on - strand at 76.658 kb on + strand at 76.671 kb on + strand at 76.671 kb on + strand at 76.672 kb on - strand at 76.673 kb on + strand at 76.720 kb on - strand, within GFF3069 at 76.804 kb on - strand, within GFF3069 at 76.837 kb on + strand, within GFF3069 at 76.906 kb on - strand, within GFF3069 at 76.908 kb on - strand, within GFF3069 at 76.931 kb on + strand, within GFF3069 at 76.932 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.941 kb on + strand, within GFF3069 at 76.970 kb on + strand, within GFF3069 at 77.019 kb on - strand, within GFF3069 at 77.019 kb on - strand, within GFF3069 at 77.076 kb on + strand, within GFF3069 at 77.121 kb on - strand, within GFF3069 at 77.298 kb on - strand, within GFF3069 at 77.298 kb on - strand, within GFF3069 at 77.334 kb on - strand, within GFF3069 at 77.429 kb on + strand, within GFF3069 at 77.444 kb on + strand, within GFF3069 at 77.445 kb on - strand, within GFF3069 at 77.451 kb on - strand, within GFF3069 at 77.600 kb on - strand, within GFF3069 at 77.688 kb on - strand, within GFF3069 at 77.757 kb on + strand at 77.800 kb on - strand at 77.800 kb on - strand at 77.922 kb on - strand at 77.928 kb on - strand at 77.928 kb on - strand at 77.961 kb on + strand at 77.962 kb on - strand at 77.963 kb on + strand at 77.966 kb on - strand at 77.966 kb on - strand at 78.135 kb on + strand, within GFF3070 at 78.148 kb on + strand, within GFF3070 at 78.260 kb on + strand, within GFF3070 at 78.260 kb on + strand, within GFF3070 at 78.283 kb on + strand, within GFF3070 at 78.283 kb on + strand, within GFF3070 at 78.284 kb on - strand, within GFF3070 at 78.500 kb on + strand, within GFF3070 at 78.501 kb on - strand, within GFF3070 at 78.618 kb on - strand, within GFF3070
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 75,600 - GFF3067 0.29 -0.1 75,605 + GFF3067 0.29 +1.2 75,606 - GFF3067 0.29 -0.5 75,693 + GFF3067 0.37 +0.2 75,809 + GFF3067 0.48 +0.2 75,852 - GFF3067 0.52 +0.4 75,867 + GFF3067 0.53 +1.6 75,868 - GFF3067 0.53 -0.3 75,897 - GFF3067 0.56 -3.1 75,900 + GFF3067 0.56 -0.6 76,000 + GFF3067 0.66 -0.6 76,001 - GFF3067 0.66 +1.1 76,044 - GFF3067 0.70 -0.1 76,110 + GFF3067 0.76 -0.4 76,127 + GFF3067 0.77 -0.3 76,128 - GFF3067 0.77 +0.2 76,210 + GFF3067 0.85 +0.2 76,210 + GFF3067 0.85 -0.5 76,210 + GFF3067 0.85 +0.3 76,211 - GFF3067 0.85 +0.7 76,291 + -0.3 76,291 + -1.1 76,332 + -0.1 76,332 + +0.4 76,332 + -0.1 76,332 + +0.4 76,333 - -0.1 76,334 + +0.6 76,611 - -0.3 76,658 + -0.1 76,671 + +0.2 76,671 + +0.4 76,672 - -0.7 76,673 + +0.5 76,720 - GFF3069 0.10 -1.2 76,804 - GFF3069 0.17 +0.2 76,837 + GFF3069 0.20 -0.3 76,906 - GFF3069 0.25 +0.3 76,908 - GFF3069 0.25 -2.6 76,931 + GFF3069 0.27 -0.2 76,932 - GFF3069 0.27 -1.4 76,935 - GFF3069 0.27 -1.1 76,935 - GFF3069 0.27 -0.3 76,935 - GFF3069 0.27 -0.9 76,941 + GFF3069 0.28 -1.1 76,970 + GFF3069 0.30 +0.0 77,019 - GFF3069 0.34 +0.4 77,019 - GFF3069 0.34 +0.1 77,076 + GFF3069 0.39 +0.7 77,121 - GFF3069 0.42 +0.5 77,298 - GFF3069 0.56 +0.0 77,298 - GFF3069 0.56 +0.1 77,334 - GFF3069 0.59 -0.7 77,429 + GFF3069 0.67 -0.7 77,444 + GFF3069 0.68 +0.1 77,445 - GFF3069 0.68 -0.3 77,451 - GFF3069 0.68 -0.2 77,600 - GFF3069 0.80 +0.1 77,688 - GFF3069 0.87 -0.0 77,757 + +0.9 77,800 - -0.7 77,800 - +0.3 77,922 - -0.5 77,928 - +0.7 77,928 - +0.6 77,961 + -0.0 77,962 - -1.3 77,963 + +1.6 77,966 - +1.9 77,966 - -0.8 78,135 + GFF3070 0.11 -0.1 78,148 + GFF3070 0.11 +0.7 78,260 + GFF3070 0.16 -0.2 78,260 + GFF3070 0.16 -0.1 78,283 + GFF3070 0.18 +0.7 78,283 + GFF3070 0.18 -0.8 78,284 - GFF3070 0.18 +0.1 78,500 + GFF3070 0.28 -1.0 78,501 - GFF3070 0.28 -0.1 78,618 - GFF3070 0.33 +0.1
Or see this region's nucleotide sequence