Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3065 and GFF3066 are separated by 27 nucleotides GFF3066 and GFF3067 are separated by 112 nucleotides
GFF3065 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 73,820 to 74,872
GFF3065
GFF3066 - FIG001341: Probable Fe(2+)-trafficking protein YggX, at 74,900 to 75,175
GFF3066
GFF3067 - Membrane-bound lytic murein transglycosylase C precursor (EC 3.2.1.-), at 75,288 to 76,373
GFF3067
Position (kb) 74
75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 74.212 kb on + strand, within GFF3065 at 74.305 kb on + strand, within GFF3065 at 74.359 kb on + strand, within GFF3065 at 74.359 kb on + strand, within GFF3065 at 74.532 kb on + strand, within GFF3065 at 74.540 kb on - strand, within GFF3065 at 74.594 kb on + strand, within GFF3065 at 74.594 kb on + strand, within GFF3065 at 74.595 kb on - strand, within GFF3065 at 74.728 kb on + strand, within GFF3065 at 74.841 kb on + strand at 74.850 kb on + strand at 74.899 kb on + strand at 74.900 kb on - strand at 74.990 kb on + strand, within GFF3066 at 74.991 kb on - strand, within GFF3066 at 75.032 kb on - strand, within GFF3066 at 75.143 kb on - strand, within GFF3066 at 75.217 kb on + strand at 75.453 kb on + strand, within GFF3067 at 75.455 kb on + strand, within GFF3067 at 75.496 kb on - strand, within GFF3067 at 75.496 kb on - strand, within GFF3067 at 75.600 kb on - strand, within GFF3067 at 75.605 kb on + strand, within GFF3067 at 75.606 kb on - strand, within GFF3067 at 75.693 kb on + strand, within GFF3067 at 75.809 kb on + strand, within GFF3067 at 75.852 kb on - strand, within GFF3067 at 75.867 kb on + strand, within GFF3067 at 75.868 kb on - strand, within GFF3067 at 75.897 kb on - strand, within GFF3067 at 75.900 kb on + strand, within GFF3067 at 76.000 kb on + strand, within GFF3067 at 76.001 kb on - strand, within GFF3067 at 76.044 kb on - strand, within GFF3067 at 76.110 kb on + strand, within GFF3067 at 76.127 kb on + strand, within GFF3067 at 76.128 kb on - strand, within GFF3067
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 74,212 + GFF3065 0.37 +0.3 74,305 + GFF3065 0.46 +0.1 74,359 + GFF3065 0.51 +0.1 74,359 + GFF3065 0.51 -0.7 74,532 + GFF3065 0.68 +0.9 74,540 - GFF3065 0.68 +0.1 74,594 + GFF3065 0.74 +0.5 74,594 + GFF3065 0.74 +0.5 74,595 - GFF3065 0.74 +0.3 74,728 + GFF3065 0.86 +3.1 74,841 + -1.6 74,850 + +0.6 74,899 + +1.1 74,900 - -0.4 74,990 + GFF3066 0.33 -0.1 74,991 - GFF3066 0.33 +0.2 75,032 - GFF3066 0.48 -0.3 75,143 - GFF3066 0.88 -0.4 75,217 + -0.7 75,453 + GFF3067 0.15 +0.1 75,455 + GFF3067 0.15 -0.7 75,496 - GFF3067 0.19 -0.9 75,496 - GFF3067 0.19 -0.3 75,600 - GFF3067 0.29 -0.1 75,605 + GFF3067 0.29 +1.2 75,606 - GFF3067 0.29 -0.5 75,693 + GFF3067 0.37 +0.2 75,809 + GFF3067 0.48 +0.2 75,852 - GFF3067 0.52 +0.4 75,867 + GFF3067 0.53 +1.6 75,868 - GFF3067 0.53 -0.3 75,897 - GFF3067 0.56 -3.1 75,900 + GFF3067 0.56 -0.6 76,000 + GFF3067 0.66 -0.6 76,001 - GFF3067 0.66 +1.1 76,044 - GFF3067 0.70 -0.1 76,110 + GFF3067 0.76 -0.4 76,127 + GFF3067 0.77 -0.3 76,128 - GFF3067 0.77 +0.2
Or see this region's nucleotide sequence