Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2628 and GFF2629 are separated by 463 nucleotides GFF2629 and GFF2630 are separated by 270 nucleotides GFF2630 and GFF2631 are separated by 116 nucleotides
GFF2628 - putative membrane protein, at 152,494 to 154,044
GFF2628
GFF2629 - Putative SAM-dependent methyltransferases, at 154,508 to 155,170
GFF2629
GFF2630 - Cytochrome b(561), at 155,441 to 155,971
GFF2630
GFF2631 - Protein ydcF, at 156,088 to 156,888
GFF2631
Position (kb)
154
155
156 Strain fitness (log2 ratio)
-1
0
1 at 153.765 kb on + strand, within GFF2628 at 153.863 kb on - strand, within GFF2628 at 154.563 kb on + strand at 154.630 kb on - strand, within GFF2629 at 154.871 kb on + strand, within GFF2629 at 154.887 kb on + strand, within GFF2629 at 155.053 kb on + strand, within GFF2629 at 155.280 kb on + strand at 155.280 kb on + strand at 155.280 kb on + strand at 155.281 kb on - strand at 155.381 kb on + strand at 155.382 kb on - strand at 155.382 kb on - strand at 155.431 kb on + strand at 155.476 kb on + strand at 155.476 kb on + strand at 155.538 kb on + strand, within GFF2630 at 155.617 kb on + strand, within GFF2630 at 155.617 kb on + strand, within GFF2630 at 155.655 kb on + strand, within GFF2630 at 155.656 kb on - strand, within GFF2630 at 155.717 kb on + strand, within GFF2630 at 155.807 kb on + strand, within GFF2630 at 155.846 kb on - strand, within GFF2630 at 155.867 kb on - strand, within GFF2630 at 156.008 kb on - strand at 156.019 kb on - strand at 156.021 kb on - strand at 156.095 kb on - strand at 156.126 kb on + strand at 156.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 153,765 + GFF2628 0.82 +0.5 153,863 - GFF2628 0.88 -0.5 154,563 + +0.2 154,630 - GFF2629 0.18 -1.7 154,871 + GFF2629 0.55 +0.0 154,887 + GFF2629 0.57 -0.2 155,053 + GFF2629 0.82 +0.0 155,280 + +0.4 155,280 + +0.6 155,280 + -0.8 155,281 - -0.3 155,381 + +0.0 155,382 - +0.2 155,382 - +0.3 155,431 + -0.3 155,476 + +0.4 155,476 + +0.8 155,538 + GFF2630 0.18 -1.1 155,617 + GFF2630 0.33 +0.8 155,617 + GFF2630 0.33 +0.2 155,655 + GFF2630 0.40 +0.0 155,656 - GFF2630 0.40 +0.1 155,717 + GFF2630 0.52 -0.0 155,807 + GFF2630 0.69 -0.7 155,846 - GFF2630 0.76 -0.2 155,867 - GFF2630 0.80 -1.4 156,008 - +0.9 156,019 - -0.6 156,021 - +0.1 156,095 - -0.9 156,126 + +0.4 156,142 + -0.2
Or see this region's nucleotide sequence