Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2289 and GFF2290 are separated by 97 nucleotides GFF2290 and GFF2291 are separated by 15 nucleotides GFF2291 and GFF2292 are separated by 89 nucleotides GFF2292 and GFF2293 are separated by 13 nucleotides GFF2293 and GFF2294 are separated by 129 nucleotides
GFF2289 - Transcriptional regulator, GntR family, at 77,945 to 78,667
GFF2289
GFF2290 - Putative periplasmic protein, at 78,765 to 78,929
GFF2290
GFF2291 - Putative periplasmic protein, at 78,945 to 79,076
GFF2291
GFF2292 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 79,166 to 79,516
GFF2292
GFF2293 - Autoinducer 2 (AI-2) kinase LsrK (EC 2.7.1.-), at 79,530 to 81,080
GFF2293
GFF2294 - LsrR, transcriptional repressor of lsr operon, at 81,210 to 82,169
GFF2294
Position (kb) 79
80
81
82 Strain fitness (log2 ratio) -2
-1
0
1
2 at 78.554 kb on - strand, within GFF2289 at 78.700 kb on + strand at 78.701 kb on - strand at 78.712 kb on - strand at 78.764 kb on + strand at 78.765 kb on - strand at 78.765 kb on - strand at 78.819 kb on - strand, within GFF2290 at 79.167 kb on + strand at 79.264 kb on - strand, within GFF2292 at 79.289 kb on + strand, within GFF2292 at 79.313 kb on - strand, within GFF2292 at 79.353 kb on - strand, within GFF2292 at 79.358 kb on - strand, within GFF2292 at 79.378 kb on + strand, within GFF2292 at 79.379 kb on - strand, within GFF2292 at 79.381 kb on + strand, within GFF2292 at 79.382 kb on - strand, within GFF2292 at 79.382 kb on - strand, within GFF2292 at 79.418 kb on + strand, within GFF2292 at 79.418 kb on + strand, within GFF2292 at 79.418 kb on + strand, within GFF2292 at 79.652 kb on + strand at 79.653 kb on - strand at 79.776 kb on + strand, within GFF2293 at 79.776 kb on + strand, within GFF2293 at 79.944 kb on + strand, within GFF2293 at 79.959 kb on - strand, within GFF2293 at 79.988 kb on + strand, within GFF2293 at 79.989 kb on - strand, within GFF2293 at 80.009 kb on - strand, within GFF2293 at 80.009 kb on - strand, within GFF2293 at 80.101 kb on + strand, within GFF2293 at 80.111 kb on + strand, within GFF2293 at 80.112 kb on - strand, within GFF2293 at 80.115 kb on + strand, within GFF2293 at 80.131 kb on - strand, within GFF2293 at 80.195 kb on + strand, within GFF2293 at 80.208 kb on + strand, within GFF2293 at 80.355 kb on + strand, within GFF2293 at 80.591 kb on + strand, within GFF2293 at 80.591 kb on + strand, within GFF2293 at 80.592 kb on - strand, within GFF2293 at 80.592 kb on - strand, within GFF2293 at 80.592 kb on - strand, within GFF2293 at 80.615 kb on + strand, within GFF2293 at 80.615 kb on + strand, within GFF2293 at 80.615 kb on + strand, within GFF2293 at 80.616 kb on - strand, within GFF2293 at 80.616 kb on - strand, within GFF2293 at 80.639 kb on - strand, within GFF2293 at 80.643 kb on - strand, within GFF2293 at 80.916 kb on - strand, within GFF2293 at 81.030 kb on + strand at 81.085 kb on + strand at 81.107 kb on + strand at 81.274 kb on + strand at 81.379 kb on - strand, within GFF2294 at 81.387 kb on - strand, within GFF2294 at 81.507 kb on + strand, within GFF2294 at 81.507 kb on + strand, within GFF2294 at 81.659 kb on + strand, within GFF2294 at 81.659 kb on + strand, within GFF2294 at 81.660 kb on - strand, within GFF2294 at 81.662 kb on + strand, within GFF2294 at 81.662 kb on + strand, within GFF2294 at 81.666 kb on + strand, within GFF2294 at 81.666 kb on + strand, within GFF2294 at 81.667 kb on - strand, within GFF2294 at 81.672 kb on - strand, within GFF2294 at 81.705 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.707 kb on - strand, within GFF2294 at 81.848 kb on + strand, within GFF2294
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 78,554 - GFF2289 0.84 -0.5 78,700 + -0.2 78,701 - -0.4 78,712 - +0.4 78,764 + +1.1 78,765 - +0.5 78,765 - +0.6 78,819 - GFF2290 0.33 +0.4 79,167 + +0.3 79,264 - GFF2292 0.28 +0.1 79,289 + GFF2292 0.35 +0.3 79,313 - GFF2292 0.42 -0.8 79,353 - GFF2292 0.53 -0.3 79,358 - GFF2292 0.55 -1.2 79,378 + GFF2292 0.60 +1.1 79,379 - GFF2292 0.61 +0.2 79,381 + GFF2292 0.61 +0.2 79,382 - GFF2292 0.62 +0.8 79,382 - GFF2292 0.62 -0.1 79,418 + GFF2292 0.72 +1.0 79,418 + GFF2292 0.72 +0.1 79,418 + GFF2292 0.72 -0.0 79,652 + +0.0 79,653 - -0.4 79,776 + GFF2293 0.16 +0.1 79,776 + GFF2293 0.16 -0.1 79,944 + GFF2293 0.27 +0.2 79,959 - GFF2293 0.28 -0.6 79,988 + GFF2293 0.30 -0.7 79,989 - GFF2293 0.30 +0.3 80,009 - GFF2293 0.31 +0.0 80,009 - GFF2293 0.31 -0.1 80,101 + GFF2293 0.37 -0.2 80,111 + GFF2293 0.37 +0.2 80,112 - GFF2293 0.38 -0.4 80,115 + GFF2293 0.38 -0.2 80,131 - GFF2293 0.39 +0.3 80,195 + GFF2293 0.43 +0.4 80,208 + GFF2293 0.44 +0.2 80,355 + GFF2293 0.53 -0.8 80,591 + GFF2293 0.68 -1.9 80,591 + GFF2293 0.68 -0.9 80,592 - GFF2293 0.68 +0.3 80,592 - GFF2293 0.68 +0.0 80,592 - GFF2293 0.68 -0.0 80,615 + GFF2293 0.70 -0.9 80,615 + GFF2293 0.70 +2.1 80,615 + GFF2293 0.70 -0.4 80,616 - GFF2293 0.70 +0.5 80,616 - GFF2293 0.70 -0.2 80,639 - GFF2293 0.72 -0.2 80,643 - GFF2293 0.72 +0.5 80,916 - GFF2293 0.89 +1.1 81,030 + +0.0 81,085 + -1.5 81,107 + -1.1 81,274 + -0.5 81,379 - GFF2294 0.18 -0.9 81,387 - GFF2294 0.18 -0.8 81,507 + GFF2294 0.31 -0.5 81,507 + GFF2294 0.31 +0.4 81,659 + GFF2294 0.47 -1.1 81,659 + GFF2294 0.47 -0.1 81,660 - GFF2294 0.47 +0.5 81,662 + GFF2294 0.47 +0.0 81,662 + GFF2294 0.47 +1.0 81,666 + GFF2294 0.47 -1.2 81,666 + GFF2294 0.47 -0.4 81,667 - GFF2294 0.48 -0.0 81,672 - GFF2294 0.48 -0.1 81,705 - GFF2294 0.52 +0.3 81,707 - GFF2294 0.52 +0.2 81,707 - GFF2294 0.52 +0.0 81,707 - GFF2294 0.52 +0.5 81,848 + GFF2294 0.66 -0.1
Or see this region's nucleotide sequence