Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1866 and GFF1867 overlap by 4 nucleotides GFF1867 and GFF1868 overlap by 1 nucleotides GFF1868 and GFF1869 are separated by 14 nucleotides
GFF1866 - O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26), at 58,066 to 59,433
GFF1866
GFF1867 - O-succinylbenzoate synthase (EC 4.2.1.113), at 59,430 to 60,392
GFF1867
GFF1868 - Naphthoate synthase (EC 4.1.3.36), at 60,392 to 61,249
GFF1868
GFF1869 - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1- carboxylate synthase (EC 4.2.99.20), at 61,264 to 62,022
GFF1869
Position (kb) 59
60
61 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 58.606 kb on - strand, within GFF1866 at 58.779 kb on + strand, within GFF1866 at 58.780 kb on - strand, within GFF1866 at 58.857 kb on + strand, within GFF1866 at 58.858 kb on - strand, within GFF1866 at 58.858 kb on - strand, within GFF1866 at 58.858 kb on - strand, within GFF1866 at 58.860 kb on + strand, within GFF1866 at 58.861 kb on - strand, within GFF1866 at 58.861 kb on - strand, within GFF1866 at 58.971 kb on + strand, within GFF1866 at 58.971 kb on + strand, within GFF1866 at 58.971 kb on + strand, within GFF1866 at 58.972 kb on - strand, within GFF1866 at 58.972 kb on - strand, within GFF1866 at 58.972 kb on - strand, within GFF1866 at 58.975 kb on + strand, within GFF1866 at 58.976 kb on - strand, within GFF1866 at 58.976 kb on - strand, within GFF1866 at 58.976 kb on - strand, within GFF1866 at 58.976 kb on - strand, within GFF1866 at 59.075 kb on - strand, within GFF1866 at 59.288 kb on - strand, within GFF1866 at 59.523 kb on - strand at 59.664 kb on - strand, within GFF1867 at 59.685 kb on - strand, within GFF1867 at 59.864 kb on - strand, within GFF1867 at 59.864 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.302 kb on - strand at 60.376 kb on - strand at 60.681 kb on + strand, within GFF1868 at 60.739 kb on + strand, within GFF1868 at 60.742 kb on - strand, within GFF1868 at 61.034 kb on - strand, within GFF1868
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 58,606 - GFF1866 0.39 -0.5 58,779 + GFF1866 0.52 +1.8 58,780 - GFF1866 0.52 +0.8 58,857 + GFF1866 0.58 +0.6 58,858 - GFF1866 0.58 +0.5 58,858 - GFF1866 0.58 +1.8 58,858 - GFF1866 0.58 +0.4 58,860 + GFF1866 0.58 +0.1 58,861 - GFF1866 0.58 +0.8 58,861 - GFF1866 0.58 +1.0 58,971 + GFF1866 0.66 -0.6 58,971 + GFF1866 0.66 -0.4 58,971 + GFF1866 0.66 -0.5 58,972 - GFF1866 0.66 +0.6 58,972 - GFF1866 0.66 -1.0 58,972 - GFF1866 0.66 +0.0 58,975 + GFF1866 0.66 +0.4 58,976 - GFF1866 0.67 -0.1 58,976 - GFF1866 0.67 +0.3 58,976 - GFF1866 0.67 +0.2 58,976 - GFF1866 0.67 -0.4 59,075 - GFF1866 0.74 +0.5 59,288 - GFF1866 0.89 +0.3 59,523 - +0.6 59,664 - GFF1867 0.24 +1.4 59,685 - GFF1867 0.26 -3.0 59,864 - GFF1867 0.45 +0.9 59,864 - GFF1867 0.45 +1.4 60,202 - GFF1867 0.80 -0.0 60,202 - GFF1867 0.80 -1.4 60,202 - GFF1867 0.80 +0.7 60,302 - +0.2 60,376 - -0.6 60,681 + GFF1868 0.34 -0.1 60,739 + GFF1868 0.40 +1.0 60,742 - GFF1868 0.41 +1.4 61,034 - GFF1868 0.75 +0.3
Or see this region's nucleotide sequence