Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1625 and GFF1626 are separated by 93 nucleotides GFF1626 and GFF1627 are separated by 67 nucleotides GFF1627 and GFF1628 are separated by 130 nucleotides GFF1628 and GFF1629 are separated by 99 nucleotides GFF1629 and GFF1630 are separated by 37 nucleotides
GFF1625 - FIG004088: inner membrane protein YebE, at 10,490 to 11,149
GFF1625
GFF1626 - FIG01200701: possible membrane protein, at 11,243 to 11,596
GFF1626
GFF1627 - DNA damage-inducible gene in SOS regulon, dependent on cyclic AMP and H-NS, at 11,664 to 11,954
GFF1627
GFF1628 - Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-), at 12,085 to 13,263
GFF1628
GFF1629 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 13,363 to 14,004
GFF1629
GFF1630 - Phosphogluconate dehydratase (EC 4.2.1.12), at 14,042 to 15,853
GFF1630
Position (kb)
12
13
14 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 11.163 kb on + strand at 11.163 kb on + strand at 11.254 kb on + strand at 11.254 kb on + strand at 11.762 kb on + strand, within GFF1627 at 11.763 kb on - strand, within GFF1627 at 11.763 kb on - strand, within GFF1627 at 11.936 kb on - strand at 11.936 kb on - strand at 11.936 kb on - strand at 11.936 kb on - strand at 11.936 kb on - strand at 12.071 kb on - strand at 12.071 kb on - strand at 12.093 kb on - strand at 12.134 kb on + strand at 12.134 kb on + strand at 12.262 kb on - strand, within GFF1628 at 12.265 kb on - strand, within GFF1628 at 12.297 kb on + strand, within GFF1628 at 12.316 kb on + strand, within GFF1628 at 12.316 kb on + strand, within GFF1628 at 12.319 kb on - strand, within GFF1628 at 12.319 kb on - strand, within GFF1628 at 12.622 kb on + strand, within GFF1628 at 12.622 kb on + strand, within GFF1628 at 12.773 kb on - strand, within GFF1628 at 12.849 kb on - strand, within GFF1628 at 13.103 kb on + strand, within GFF1628 at 13.103 kb on + strand, within GFF1628 at 13.146 kb on + strand at 13.181 kb on + strand at 13.261 kb on + strand at 13.626 kb on - strand, within GFF1629 at 13.626 kb on - strand, within GFF1629 at 13.659 kb on + strand, within GFF1629 at 13.660 kb on - strand, within GFF1629 at 13.681 kb on - strand, within GFF1629 at 13.861 kb on + strand, within GFF1629 at 13.861 kb on + strand, within GFF1629 at 13.940 kb on - strand at 13.940 kb on - strand at 14.031 kb on - strand at 14.031 kb on - strand at 14.150 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 11,163 + -0.6 11,163 + -0.6 11,254 + +0.4 11,254 + -1.6 11,762 + GFF1627 0.34 -0.6 11,763 - GFF1627 0.34 +0.3 11,763 - GFF1627 0.34 -0.4 11,936 - +0.2 11,936 - +0.7 11,936 - +1.1 11,936 - +0.5 11,936 - -1.0 12,071 - -0.6 12,071 - +0.5 12,093 - -0.3 12,134 + +0.9 12,134 + -1.2 12,262 - GFF1628 0.15 -0.4 12,265 - GFF1628 0.15 -0.5 12,297 + GFF1628 0.18 -2.8 12,316 + GFF1628 0.20 +0.1 12,316 + GFF1628 0.20 +0.7 12,319 - GFF1628 0.20 +1.2 12,319 - GFF1628 0.20 +0.1 12,622 + GFF1628 0.46 +1.2 12,622 + GFF1628 0.46 +0.7 12,773 - GFF1628 0.58 -0.4 12,849 - GFF1628 0.65 +0.6 13,103 + GFF1628 0.86 -0.1 13,103 + GFF1628 0.86 -3.1 13,146 + +0.8 13,181 + -0.1 13,261 + -1.0 13,626 - GFF1629 0.41 +0.3 13,626 - GFF1629 0.41 -0.1 13,659 + GFF1629 0.46 -0.4 13,660 - GFF1629 0.46 +0.3 13,681 - GFF1629 0.50 -0.2 13,861 + GFF1629 0.78 -0.3 13,861 + GFF1629 0.78 -0.3 13,940 - +0.6 13,940 - -1.6 14,031 - +0.2 14,031 - -0.7 14,150 - -0.1
Or see this region's nucleotide sequence