Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1548 and GFF1549 are separated by 41 nucleotides GFF1549 and GFF1550 are separated by 343 nucleotides
GFF1548 - Periplasmic esterase IroE, at 10,794 to 11,729
GFF1548
GFF1549 - Outer Membrane Siderophore Receptor IroN, at 11,771 to 13,945
GFF1549
GFF1550 - hypothetical protein, at 14,289 to 14,432
GFF1550
Position (kb)
11
12
13
14 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 10.798 kb on - strand at 10.798 kb on - strand at 10.930 kb on - strand, within GFF1548 at 11.038 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.040 kb on + strand, within GFF1548 at 11.041 kb on - strand, within GFF1548 at 11.041 kb on - strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.042 kb on + strand, within GFF1548 at 11.145 kb on - strand, within GFF1548 at 11.175 kb on + strand, within GFF1548 at 11.176 kb on - strand, within GFF1548 at 11.176 kb on - strand, within GFF1548 at 11.176 kb on - strand, within GFF1548 at 11.285 kb on + strand, within GFF1548 at 11.286 kb on - strand, within GFF1548 at 11.352 kb on + strand, within GFF1548 at 11.402 kb on + strand, within GFF1548 at 11.402 kb on + strand, within GFF1548 at 11.403 kb on - strand, within GFF1548 at 11.406 kb on + strand, within GFF1548 at 11.407 kb on - strand, within GFF1548 at 11.410 kb on - strand, within GFF1548 at 11.410 kb on - strand, within GFF1548 at 11.439 kb on - strand, within GFF1548 at 11.462 kb on + strand, within GFF1548 at 11.507 kb on - strand, within GFF1548 at 11.796 kb on + strand at 11.800 kb on - strand at 11.819 kb on - strand at 11.821 kb on - strand at 11.821 kb on - strand at 11.821 kb on - strand at 11.824 kb on + strand at 11.825 kb on - strand at 11.910 kb on + strand at 12.018 kb on + strand, within GFF1549 at 12.019 kb on - strand, within GFF1549 at 12.076 kb on + strand, within GFF1549 at 12.076 kb on + strand, within GFF1549 at 12.077 kb on - strand, within GFF1549 at 12.144 kb on + strand, within GFF1549 at 12.145 kb on - strand, within GFF1549 at 12.145 kb on - strand, within GFF1549 at 12.157 kb on + strand, within GFF1549 at 12.248 kb on + strand, within GFF1549 at 12.248 kb on + strand, within GFF1549 at 12.436 kb on + strand, within GFF1549 at 12.473 kb on + strand, within GFF1549 at 12.474 kb on - strand, within GFF1549 at 12.477 kb on + strand, within GFF1549 at 12.649 kb on + strand, within GFF1549 at 12.706 kb on + strand, within GFF1549 at 12.767 kb on - strand, within GFF1549 at 12.831 kb on - strand, within GFF1549 at 12.910 kb on + strand, within GFF1549 at 12.913 kb on + strand, within GFF1549 at 12.913 kb on + strand, within GFF1549 at 12.914 kb on - strand, within GFF1549 at 12.975 kb on + strand, within GFF1549 at 12.975 kb on + strand, within GFF1549 at 13.199 kb on - strand, within GFF1549 at 13.261 kb on + strand, within GFF1549 at 13.262 kb on - strand, within GFF1549 at 13.321 kb on + strand, within GFF1549 at 13.399 kb on + strand, within GFF1549 at 13.402 kb on - strand, within GFF1549 at 13.517 kb on - strand, within GFF1549 at 13.582 kb on + strand, within GFF1549 at 13.584 kb on + strand, within GFF1549 at 13.631 kb on + strand, within GFF1549 at 13.735 kb on + strand at 13.883 kb on + strand at 13.892 kb on - strand at 13.903 kb on + strand at 13.960 kb on + strand at 13.960 kb on + strand at 13.960 kb on + strand at 14.249 kb on + strand at 14.271 kb on - strand at 14.278 kb on + strand at 14.428 kb on + strand at 14.429 kb on - strand at 14.534 kb on - strand at 14.573 kb on + strand at 14.573 kb on + strand at 14.692 kb on - strand at 14.700 kb on - strand at 14.775 kb on - strand at 14.777 kb on - strand at 14.788 kb on + strand at 14.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 10,798 - +0.1 10,798 - +0.2 10,930 - GFF1548 0.15 -1.5 11,038 + GFF1548 0.26 +0.5 11,040 + GFF1548 0.26 -0.0 11,040 + GFF1548 0.26 -1.3 11,040 + GFF1548 0.26 +0.1 11,040 + GFF1548 0.26 -0.3 11,040 + GFF1548 0.26 +0.8 11,040 + GFF1548 0.26 -0.0 11,041 - GFF1548 0.26 -0.2 11,041 - GFF1548 0.26 +0.4 11,042 + GFF1548 0.26 +1.0 11,042 + GFF1548 0.26 -0.3 11,042 + GFF1548 0.26 +0.2 11,042 + GFF1548 0.26 +0.5 11,042 + GFF1548 0.26 -0.3 11,145 - GFF1548 0.38 +0.3 11,175 + GFF1548 0.41 +0.4 11,176 - GFF1548 0.41 +0.5 11,176 - GFF1548 0.41 -0.1 11,176 - GFF1548 0.41 +0.3 11,285 + GFF1548 0.52 -0.3 11,286 - GFF1548 0.53 +0.2 11,352 + GFF1548 0.60 -1.1 11,402 + GFF1548 0.65 -0.4 11,402 + GFF1548 0.65 -1.6 11,403 - GFF1548 0.65 -0.2 11,406 + GFF1548 0.65 +0.2 11,407 - GFF1548 0.65 +0.8 11,410 - GFF1548 0.66 -0.4 11,410 - GFF1548 0.66 +0.3 11,439 - GFF1548 0.69 +0.1 11,462 + GFF1548 0.71 +0.8 11,507 - GFF1548 0.76 +0.3 11,796 + -0.2 11,800 - +0.4 11,819 - -0.2 11,821 - +0.3 11,821 - +0.2 11,821 - -0.5 11,824 + +0.6 11,825 - +0.1 11,910 + -0.2 12,018 + GFF1549 0.11 -0.3 12,019 - GFF1549 0.11 -0.4 12,076 + GFF1549 0.14 -1.0 12,076 + GFF1549 0.14 -0.2 12,077 - GFF1549 0.14 -1.1 12,144 + GFF1549 0.17 -1.2 12,145 - GFF1549 0.17 -0.7 12,145 - GFF1549 0.17 +0.0 12,157 + GFF1549 0.18 -1.0 12,248 + GFF1549 0.22 -0.8 12,248 + GFF1549 0.22 -0.8 12,436 + GFF1549 0.31 +0.0 12,473 + GFF1549 0.32 -0.5 12,474 - GFF1549 0.32 -0.2 12,477 + GFF1549 0.32 -0.7 12,649 + GFF1549 0.40 +0.0 12,706 + GFF1549 0.43 -0.5 12,767 - GFF1549 0.46 -0.1 12,831 - GFF1549 0.49 +1.4 12,910 + GFF1549 0.52 +0.2 12,913 + GFF1549 0.53 -0.2 12,913 + GFF1549 0.53 +0.1 12,914 - GFF1549 0.53 -0.7 12,975 + GFF1549 0.55 +0.1 12,975 + GFF1549 0.55 -0.1 13,199 - GFF1549 0.66 +0.2 13,261 + GFF1549 0.69 -0.3 13,262 - GFF1549 0.69 +0.3 13,321 + GFF1549 0.71 -0.1 13,399 + GFF1549 0.75 +0.7 13,402 - GFF1549 0.75 +0.2 13,517 - GFF1549 0.80 -2.8 13,582 + GFF1549 0.83 -0.4 13,584 + GFF1549 0.83 +0.2 13,631 + GFF1549 0.86 -0.2 13,735 + -0.1 13,883 + -0.3 13,892 - -0.8 13,903 + -1.2 13,960 + -0.0 13,960 + +0.2 13,960 + -0.5 14,249 + +0.1 14,271 - -0.4 14,278 + -0.3 14,428 + -1.0 14,429 - +0.5 14,534 - +0.4 14,573 + -1.2 14,573 + +0.1 14,692 - +0.1 14,700 - -0.0 14,775 - +0.4 14,777 - +1.1 14,788 + +0.2 14,789 - -1.1
Or see this region's nucleotide sequence