Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1212 and GFF1213 are separated by 83 nucleotides GFF1213 and GFF1214 are separated by 27 nucleotides GFF1214 and GFF1215 are separated by 274 nucleotides
GFF1212 - Kappa-fimbriae usher protein, at 51,156 to 53,564
GFF1212
GFF1213 - hypothetical protein, at 53,648 to 53,764
GFF1213
GFF1214 - Kappa-fimbriae major subunit, at 53,792 to 54,310
GFF1214
GFF1215 - Kappa-fimbriae regulatory protein, at 54,585 to 54,887
GFF1215
Position (kb) 53
54
55 Strain fitness (log2 ratio) -1
0
1
2 at 52.838 kb on - strand, within GFF1212 at 52.985 kb on + strand, within GFF1212 at 52.985 kb on + strand, within GFF1212 at 52.986 kb on - strand, within GFF1212 at 53.036 kb on - strand, within GFF1212 at 53.081 kb on - strand, within GFF1212 at 53.107 kb on + strand, within GFF1212 at 53.108 kb on - strand, within GFF1212 at 53.203 kb on + strand, within GFF1212 at 53.204 kb on - strand, within GFF1212 at 53.224 kb on + strand, within GFF1212 at 53.224 kb on + strand, within GFF1212 at 53.224 kb on + strand, within GFF1212 at 53.225 kb on - strand, within GFF1212 at 53.225 kb on - strand, within GFF1212 at 53.258 kb on - strand, within GFF1212 at 53.395 kb on + strand at 53.395 kb on + strand at 53.395 kb on + strand at 53.396 kb on - strand at 53.396 kb on - strand at 53.396 kb on - strand at 53.396 kb on - strand at 53.430 kb on + strand at 53.969 kb on + strand, within GFF1214 at 53.983 kb on + strand, within GFF1214 at 53.983 kb on + strand, within GFF1214 at 54.284 kb on - strand at 54.676 kb on + strand, within GFF1215 at 54.719 kb on - strand, within GFF1215 at 54.764 kb on + strand, within GFF1215 at 55.271 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 52,838 - GFF1212 0.70 +0.3 52,985 + GFF1212 0.76 -0.0 52,985 + GFF1212 0.76 +0.4 52,986 - GFF1212 0.76 -0.4 53,036 - GFF1212 0.78 -0.1 53,081 - GFF1212 0.80 +0.1 53,107 + GFF1212 0.81 +0.4 53,108 - GFF1212 0.81 -0.3 53,203 + GFF1212 0.85 +2.1 53,204 - GFF1212 0.85 +1.0 53,224 + GFF1212 0.86 -0.1 53,224 + GFF1212 0.86 -0.3 53,224 + GFF1212 0.86 +0.3 53,225 - GFF1212 0.86 -0.9 53,225 - GFF1212 0.86 -0.2 53,258 - GFF1212 0.87 +0.6 53,395 + +0.3 53,395 + +0.2 53,395 + -0.6 53,396 - +0.2 53,396 - +0.3 53,396 - -0.2 53,396 - -0.5 53,430 + -0.4 53,969 + GFF1214 0.34 -0.9 53,983 + GFF1214 0.37 -0.2 53,983 + GFF1214 0.37 -0.5 54,284 - +0.7 54,676 + GFF1215 0.30 -0.6 54,719 - GFF1215 0.44 -0.3 54,764 + GFF1215 0.59 +0.1 55,271 + -1.6
Or see this region's nucleotide sequence