Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1033 and GFF1034 are separated by 21 nucleotides GFF1034 and GFF1035 are separated by 91 nucleotides GFF1035 and GFF1036 are separated by 191 nucleotides GFF1036 and GFF1037 are separated by 75 nucleotides
GFF1033 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 216,153 to 216,449
GFF1033
GFF1034 - '5'-nucleotidase YjjG (EC 3.1.3.5)' transl_table=11, at 216,471 to 217,148
GFF1034
GFF1035 - Peptide chain release factor 3, at 217,240 to 218,829
GFF1035
GFF1036 - hypothetical protein, at 219,021 to 219,155
GFF1036
GFF1037 - Osmotically inducible protein OsmY, at 219,231 to 219,848
GFF1037
Position (kb)
217
218
219 Strain fitness (log2 ratio)
-2
-1
0
1 at 216.248 kb on + strand, within GFF1033 at 216.296 kb on - strand, within GFF1033 at 216.296 kb on - strand, within GFF1033 at 216.314 kb on - strand, within GFF1033 at 216.345 kb on + strand, within GFF1033 at 216.387 kb on + strand, within GFF1033 at 216.440 kb on - strand at 216.448 kb on - strand at 216.467 kb on - strand at 216.516 kb on - strand at 216.628 kb on - strand, within GFF1034 at 216.652 kb on + strand, within GFF1034 at 216.728 kb on + strand, within GFF1034 at 216.729 kb on - strand, within GFF1034 at 216.782 kb on + strand, within GFF1034 at 216.783 kb on - strand, within GFF1034 at 216.783 kb on - strand, within GFF1034 at 216.804 kb on - strand, within GFF1034 at 216.815 kb on + strand, within GFF1034 at 216.815 kb on + strand, within GFF1034 at 216.818 kb on + strand, within GFF1034 at 216.818 kb on + strand, within GFF1034 at 216.838 kb on + strand, within GFF1034 at 216.986 kb on - strand, within GFF1034 at 217.140 kb on - strand at 217.294 kb on - strand at 217.377 kb on - strand at 217.441 kb on - strand, within GFF1035 at 217.534 kb on - strand, within GFF1035 at 217.537 kb on + strand, within GFF1035 at 217.537 kb on + strand, within GFF1035 at 217.612 kb on - strand, within GFF1035 at 217.777 kb on - strand, within GFF1035 at 217.795 kb on + strand, within GFF1035 at 217.796 kb on - strand, within GFF1035 at 217.796 kb on - strand, within GFF1035 at 217.810 kb on + strand, within GFF1035 at 217.810 kb on + strand, within GFF1035 at 217.974 kb on - strand, within GFF1035 at 218.040 kb on + strand, within GFF1035 at 218.213 kb on - strand, within GFF1035 at 218.213 kb on - strand, within GFF1035 at 218.283 kb on + strand, within GFF1035 at 218.626 kb on + strand, within GFF1035 at 218.626 kb on + strand, within GFF1035 at 218.694 kb on + strand at 218.701 kb on - strand at 218.742 kb on + strand at 218.742 kb on + strand at 218.746 kb on + strand at 218.747 kb on - strand at 218.747 kb on - strand at 218.747 kb on - strand at 219.040 kb on + strand, within GFF1036 at 219.067 kb on + strand, within GFF1036 at 219.073 kb on + strand, within GFF1036 at 219.203 kb on + strand at 219.215 kb on - strand at 219.215 kb on - strand at 219.217 kb on - strand at 219.236 kb on + strand at 219.236 kb on + strand at 219.381 kb on - strand, within GFF1037 at 219.462 kb on - strand, within GFF1037 at 219.614 kb on + strand, within GFF1037
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 216,248 + GFF1033 0.32 -0.1 216,296 - GFF1033 0.48 -2.7 216,296 - GFF1033 0.48 +0.3 216,314 - GFF1033 0.54 +0.6 216,345 + GFF1033 0.65 +0.0 216,387 + GFF1033 0.79 -0.1 216,440 - -1.2 216,448 - -0.1 216,467 - +0.2 216,516 - +1.0 216,628 - GFF1034 0.23 -0.1 216,652 + GFF1034 0.27 -0.2 216,728 + GFF1034 0.38 -0.2 216,729 - GFF1034 0.38 -0.7 216,782 + GFF1034 0.46 -1.1 216,783 - GFF1034 0.46 +0.6 216,783 - GFF1034 0.46 +0.2 216,804 - GFF1034 0.49 +0.7 216,815 + GFF1034 0.51 +0.5 216,815 + GFF1034 0.51 +0.6 216,818 + GFF1034 0.51 +0.2 216,818 + GFF1034 0.51 +0.4 216,838 + GFF1034 0.54 +1.0 216,986 - GFF1034 0.76 +0.3 217,140 - -0.0 217,294 - -0.1 217,377 - -0.1 217,441 - GFF1035 0.13 +0.4 217,534 - GFF1035 0.18 +0.2 217,537 + GFF1035 0.19 +0.6 217,537 + GFF1035 0.19 +0.4 217,612 - GFF1035 0.23 -0.2 217,777 - GFF1035 0.34 -0.2 217,795 + GFF1035 0.35 +0.1 217,796 - GFF1035 0.35 -0.1 217,796 - GFF1035 0.35 +1.0 217,810 + GFF1035 0.36 -0.4 217,810 + GFF1035 0.36 +1.0 217,974 - GFF1035 0.46 -0.2 218,040 + GFF1035 0.50 +0.1 218,213 - GFF1035 0.61 +0.1 218,213 - GFF1035 0.61 -0.2 218,283 + GFF1035 0.66 +0.7 218,626 + GFF1035 0.87 +0.3 218,626 + GFF1035 0.87 +0.9 218,694 + -0.6 218,701 - +0.6 218,742 + -0.2 218,742 + +0.1 218,746 + +0.4 218,747 - -0.4 218,747 - +0.3 218,747 - +0.2 219,040 + GFF1036 0.14 +0.9 219,067 + GFF1036 0.34 -0.0 219,073 + GFF1036 0.39 +0.5 219,203 + -0.6 219,215 - +0.2 219,215 - -0.1 219,217 - -1.0 219,236 + -0.8 219,236 + +0.2 219,381 - GFF1037 0.24 -2.2 219,462 - GFF1037 0.37 +0.1 219,614 + GFF1037 0.62 -0.5
Or see this region's nucleotide sequence