Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF978 and GFF979 are separated by 397 nucleotides GFF979 and GFF980 are separated by 0 nucleotides
GFF978 - Dienelactone hydrolase family, at 163,380 to 164,054
GFF978
GFF979 - Uxu operon transcriptional regulator, at 164,452 to 165,177
GFF979
GFF980 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 165,178 to 166,191
GFF980
Position (kb) 164
165
166 Strain fitness (log2 ratio) -2
-1
0
1
2 at 163.484 kb on + strand, within GFF978 at 163.517 kb on - strand, within GFF978 at 163.524 kb on - strand, within GFF978 at 163.588 kb on + strand, within GFF978 at 163.698 kb on + strand, within GFF978 at 163.724 kb on + strand, within GFF978 at 163.724 kb on + strand, within GFF978 at 163.724 kb on + strand, within GFF978 at 163.725 kb on - strand, within GFF978 at 163.725 kb on - strand, within GFF978 at 163.725 kb on - strand, within GFF978 at 163.732 kb on - strand, within GFF978 at 163.773 kb on + strand, within GFF978 at 163.773 kb on + strand, within GFF978 at 163.883 kb on + strand, within GFF978 at 163.884 kb on - strand, within GFF978 at 163.885 kb on + strand, within GFF978 at 163.885 kb on + strand, within GFF978 at 163.885 kb on + strand, within GFF978 at 163.886 kb on - strand, within GFF978 at 163.886 kb on - strand, within GFF978 at 163.886 kb on - strand, within GFF978 at 163.886 kb on - strand, within GFF978 at 163.940 kb on + strand, within GFF978 at 164.049 kb on + strand at 164.049 kb on + strand at 164.050 kb on - strand at 164.104 kb on - strand at 164.118 kb on - strand at 164.351 kb on + strand at 164.361 kb on - strand at 164.361 kb on - strand at 164.361 kb on - strand at 164.545 kb on - strand, within GFF979 at 164.618 kb on - strand, within GFF979 at 164.674 kb on - strand, within GFF979 at 164.773 kb on - strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.890 kb on - strand, within GFF979 at 164.890 kb on - strand, within GFF979 at 164.958 kb on + strand, within GFF979 at 165.125 kb on - strand at 165.137 kb on - strand at 165.182 kb on - strand at 165.190 kb on + strand at 165.218 kb on - strand at 165.227 kb on - strand at 165.460 kb on - strand, within GFF980 at 165.521 kb on + strand, within GFF980 at 165.577 kb on - strand, within GFF980 at 165.774 kb on + strand, within GFF980 at 165.774 kb on + strand, within GFF980 at 165.798 kb on - strand, within GFF980 at 165.798 kb on - strand, within GFF980 at 165.857 kb on - strand, within GFF980 at 165.862 kb on - strand, within GFF980 at 165.894 kb on + strand, within GFF980 at 165.903 kb on - strand, within GFF980 at 166.032 kb on + strand, within GFF980 at 166.076 kb on + strand, within GFF980 at 166.077 kb on - strand, within GFF980 at 166.077 kb on - strand, within GFF980
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 163,484 + GFF978 0.15 +1.9 163,517 - GFF978 0.20 -0.3 163,524 - GFF978 0.21 -0.3 163,588 + GFF978 0.31 +1.1 163,698 + GFF978 0.47 +0.3 163,724 + GFF978 0.51 +0.2 163,724 + GFF978 0.51 -0.2 163,724 + GFF978 0.51 -0.4 163,725 - GFF978 0.51 -1.0 163,725 - GFF978 0.51 +0.4 163,725 - GFF978 0.51 +0.1 163,732 - GFF978 0.52 -1.1 163,773 + GFF978 0.58 +0.4 163,773 + GFF978 0.58 -0.1 163,883 + GFF978 0.75 -0.5 163,884 - GFF978 0.75 -0.9 163,885 + GFF978 0.75 -0.1 163,885 + GFF978 0.75 -0.0 163,885 + GFF978 0.75 +0.4 163,886 - GFF978 0.75 -1.9 163,886 - GFF978 0.75 -1.0 163,886 - GFF978 0.75 -0.1 163,886 - GFF978 0.75 -0.7 163,940 + GFF978 0.83 +0.4 164,049 + -0.4 164,049 + -0.1 164,050 - -0.1 164,104 - -1.5 164,118 - -0.3 164,351 + -0.7 164,361 - -0.2 164,361 - -1.3 164,361 - -0.2 164,545 - GFF979 0.13 -1.7 164,618 - GFF979 0.23 -0.2 164,674 - GFF979 0.31 -0.1 164,773 - GFF979 0.44 -1.6 164,889 + GFF979 0.60 +0.9 164,889 + GFF979 0.60 +0.1 164,889 + GFF979 0.60 -0.1 164,890 - GFF979 0.60 -1.5 164,890 - GFF979 0.60 -1.1 164,958 + GFF979 0.70 -0.9 165,125 - -0.5 165,137 - -1.8 165,182 - +0.2 165,190 + -1.1 165,218 - -2.0 165,227 - +0.1 165,460 - GFF980 0.28 +0.9 165,521 + GFF980 0.34 +0.1 165,577 - GFF980 0.39 -0.6 165,774 + GFF980 0.59 -0.3 165,774 + GFF980 0.59 -0.1 165,798 - GFF980 0.61 +0.1 165,798 - GFF980 0.61 +0.0 165,857 - GFF980 0.67 +0.2 165,862 - GFF980 0.67 -1.4 165,894 + GFF980 0.71 -0.1 165,903 - GFF980 0.71 -0.5 166,032 + GFF980 0.84 +0.1 166,076 + GFF980 0.89 +0.1 166,077 - GFF980 0.89 +0.4 166,077 - GFF980 0.89 +0.4
Or see this region's nucleotide sequence