Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF955 and GFF956 are separated by 216 nucleotides GFF956 and GFF957 are separated by 27 nucleotides
GFF955 - L-idonate 5-dehydrogenase (EC 1.1.1.264), at 131,315 to 132,346
GFF955
GFF956 - Gluconokinase (EC 2.7.1.12), at 132,563 to 133,093
GFF956
GFF957 - Alcohol dehydrogenase (EC 1.1.1.1), at 133,121 to 134,140
GFF957
Position (kb)
132
133
134 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 131.754 kb on - strand, within GFF955 at 131.788 kb on + strand, within GFF955 at 131.824 kb on + strand, within GFF955 at 132.406 kb on - strand at 132.417 kb on - strand at 132.844 kb on + strand, within GFF956 at 132.862 kb on - strand, within GFF956 at 132.888 kb on - strand, within GFF956 at 132.896 kb on - strand, within GFF956 at 132.932 kb on - strand, within GFF956 at 133.016 kb on - strand, within GFF956 at 133.059 kb on + strand at 133.136 kb on + strand at 133.269 kb on + strand, within GFF957 at 133.269 kb on + strand, within GFF957 at 133.381 kb on - strand, within GFF957 at 133.399 kb on - strand, within GFF957 at 133.399 kb on - strand, within GFF957 at 133.432 kb on + strand, within GFF957 at 133.645 kb on + strand, within GFF957 at 133.645 kb on + strand, within GFF957 at 133.646 kb on - strand, within GFF957 at 133.646 kb on - strand, within GFF957 at 133.676 kb on - strand, within GFF957 at 133.676 kb on - strand, within GFF957 at 133.689 kb on - strand, within GFF957 at 133.689 kb on - strand, within GFF957 at 133.712 kb on - strand, within GFF957 at 133.715 kb on + strand, within GFF957 at 133.716 kb on - strand, within GFF957 at 133.716 kb on - strand, within GFF957 at 133.817 kb on - strand, within GFF957 at 133.906 kb on + strand, within GFF957 at 134.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 131,754 - GFF955 0.43 -1.9 131,788 + GFF955 0.46 -0.1 131,824 + GFF955 0.49 -0.4 132,406 - -0.2 132,417 - -0.4 132,844 + GFF956 0.53 -0.7 132,862 - GFF956 0.56 -2.1 132,888 - GFF956 0.61 -0.9 132,896 - GFF956 0.63 -0.1 132,932 - GFF956 0.69 -1.3 133,016 - GFF956 0.85 +1.8 133,059 + -2.6 133,136 + +0.1 133,269 + GFF957 0.15 +1.2 133,269 + GFF957 0.15 +0.5 133,381 - GFF957 0.25 -0.1 133,399 - GFF957 0.27 -0.1 133,399 - GFF957 0.27 -0.1 133,432 + GFF957 0.30 -0.1 133,645 + GFF957 0.51 +0.1 133,645 + GFF957 0.51 +0.5 133,646 - GFF957 0.51 -0.3 133,646 - GFF957 0.51 -0.6 133,676 - GFF957 0.54 -0.3 133,676 - GFF957 0.54 -0.1 133,689 - GFF957 0.56 -0.1 133,689 - GFF957 0.56 +0.5 133,712 - GFF957 0.58 -0.3 133,715 + GFF957 0.58 -0.4 133,716 - GFF957 0.58 -0.4 133,716 - GFF957 0.58 -0.7 133,817 - GFF957 0.68 +0.5 133,906 + GFF957 0.77 +0.8 134,080 - +0.4
Or see this region's nucleotide sequence