Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4475 and GFF4476 are separated by 127 nucleotides GFF4476 and GFF4477 are separated by 66 nucleotides
GFF4475 - NtrC family Transcriptional regulator, ATPase domain, at 259,631 to 262,273
GFF4475
GFF4476 - Putative phosphosugar isomerase, at 262,401 to 263,387
GFF4476
GFF4477 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 263,454 to 264,515
GFF4477
Position (kb) 262
263
264 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 261.847 kb on - strand, within GFF4475 at 261.858 kb on + strand, within GFF4475 at 262.280 kb on + strand at 262.400 kb on - strand at 262.473 kb on - strand at 262.652 kb on + strand, within GFF4476 at 262.652 kb on + strand, within GFF4476 at 262.711 kb on - strand, within GFF4476 at 262.711 kb on - strand, within GFF4476 at 262.749 kb on - strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.754 kb on + strand, within GFF4476 at 262.801 kb on + strand, within GFF4476 at 262.802 kb on - strand, within GFF4476 at 262.854 kb on + strand, within GFF4476 at 262.928 kb on - strand, within GFF4476 at 263.027 kb on - strand, within GFF4476 at 263.038 kb on + strand, within GFF4476 at 263.217 kb on - strand, within GFF4476 at 263.727 kb on - strand, within GFF4477 at 263.758 kb on + strand, within GFF4477 at 263.759 kb on - strand, within GFF4477 at 264.117 kb on - strand, within GFF4477 at 264.122 kb on + strand, within GFF4477 at 264.125 kb on - strand, within GFF4477 at 264.125 kb on - strand, within GFF4477 at 264.321 kb on + strand, within GFF4477
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 261,847 - GFF4475 0.84 -2.9 261,858 + GFF4475 0.84 +0.1 262,280 + +0.6 262,400 - -0.0 262,473 - -0.1 262,652 + GFF4476 0.25 -0.3 262,652 + GFF4476 0.25 -1.0 262,711 - GFF4476 0.31 +0.5 262,711 - GFF4476 0.31 -0.8 262,749 - GFF4476 0.35 -0.8 262,754 + GFF4476 0.36 -0.5 262,754 + GFF4476 0.36 +0.1 262,754 + GFF4476 0.36 +0.3 262,754 + GFF4476 0.36 -0.2 262,801 + GFF4476 0.41 -0.0 262,802 - GFF4476 0.41 +0.2 262,854 + GFF4476 0.46 +0.1 262,928 - GFF4476 0.53 +0.2 263,027 - GFF4476 0.63 -0.2 263,038 + GFF4476 0.65 -0.2 263,217 - GFF4476 0.83 -0.4 263,727 - GFF4477 0.26 -0.5 263,758 + GFF4477 0.29 -0.5 263,759 - GFF4477 0.29 -0.3 264,117 - GFF4477 0.62 +0.8 264,122 + GFF4477 0.63 +0.1 264,125 - GFF4477 0.63 +0.1 264,125 - GFF4477 0.63 +0.5 264,321 + GFF4477 0.82 -0.6
Or see this region's nucleotide sequence