Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3824 and GFF3825 are separated by 83 nucleotides GFF3825 and GFF3826 are separated by 277 nucleotides GFF3826 and GFF3827 are separated by 278 nucleotides
GFF3824 - Methionyl-tRNA synthetase (EC 6.1.1.10), at 93,080 to 95,194
GFF3824
GFF3825 - Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like, at 95,278 to 96,387
GFF3825
GFF3826 - FIG00510335: hypothetical protein, at 96,665 to 96,946
GFF3826
GFF3827 - probable fimbrial chain protein stcA, at 97,225 to 97,755
GFF3827
Position (kb) 95
96
97 Strain fitness (log2 ratio) -1
0
1 at 95.154 kb on - strand at 95.233 kb on + strand at 95.419 kb on - strand, within GFF3825 at 95.419 kb on - strand, within GFF3825 at 95.419 kb on - strand, within GFF3825 at 95.505 kb on + strand, within GFF3825 at 95.508 kb on + strand, within GFF3825 at 95.542 kb on + strand, within GFF3825 at 95.784 kb on + strand, within GFF3825 at 95.964 kb on + strand, within GFF3825 at 96.114 kb on + strand, within GFF3825 at 96.115 kb on - strand, within GFF3825 at 96.115 kb on - strand, within GFF3825 at 96.115 kb on - strand, within GFF3825 at 96.228 kb on + strand, within GFF3825 at 96.228 kb on + strand, within GFF3825 at 96.302 kb on - strand at 96.386 kb on - strand at 96.434 kb on - strand at 96.687 kb on + strand at 96.693 kb on + strand at 96.829 kb on + strand, within GFF3826
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 95,154 - -0.5 95,233 + -0.3 95,419 - GFF3825 0.13 +1.4 95,419 - GFF3825 0.13 -0.0 95,419 - GFF3825 0.13 -0.7 95,505 + GFF3825 0.20 +1.0 95,508 + GFF3825 0.21 -0.5 95,542 + GFF3825 0.24 +0.8 95,784 + GFF3825 0.46 +0.6 95,964 + GFF3825 0.62 +0.6 96,114 + GFF3825 0.75 +0.1 96,115 - GFF3825 0.75 +0.4 96,115 - GFF3825 0.75 -0.1 96,115 - GFF3825 0.75 +0.6 96,228 + GFF3825 0.86 -0.3 96,228 + GFF3825 0.86 +1.3 96,302 - +0.2 96,386 - -0.2 96,434 - -0.2 96,687 + +0.4 96,693 + -0.5 96,829 + GFF3826 0.58 +0.1
Or see this region's nucleotide sequence