Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3800 and GFF3801 are separated by 15 nucleotides GFF3801 and GFF3802 are separated by 8 nucleotides GFF3802 and GFF3803 are separated by 14 nucleotides
GFF3800 - Gentisate 1,2-dioxygenase (EC 1.13.11.4), at 69,466 to 70,503
GFF3800
GFF3801 - Fumarylacetoacetase (EC 3.7.1.2), at 70,519 to 71,220
GFF3801
GFF3802 - Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18), at 71,229 to 71,873
GFF3802
GFF3803 - Putative n-hydroxybenzoate hydroxylase, at 71,888 to 73,081
GFF3803
Position (kb)
70
71
72 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 69.520 kb on - strand at 69.632 kb on + strand, within GFF3800 at 69.740 kb on - strand, within GFF3800 at 69.749 kb on + strand, within GFF3800 at 69.813 kb on + strand, within GFF3800 at 70.045 kb on + strand, within GFF3800 at 70.046 kb on - strand, within GFF3800 at 70.123 kb on + strand, within GFF3800 at 70.308 kb on + strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.309 kb on - strand, within GFF3800 at 70.312 kb on + strand, within GFF3800 at 70.312 kb on + strand, within GFF3800 at 70.501 kb on + strand at 70.528 kb on + strand at 70.621 kb on + strand, within GFF3801 at 70.622 kb on - strand, within GFF3801 at 70.760 kb on - strand, within GFF3801 at 70.765 kb on + strand, within GFF3801 at 70.766 kb on - strand, within GFF3801 at 70.794 kb on + strand, within GFF3801 at 70.835 kb on - strand, within GFF3801 at 70.959 kb on - strand, within GFF3801 at 70.981 kb on - strand, within GFF3801 at 70.999 kb on - strand, within GFF3801 at 71.080 kb on + strand, within GFF3801 at 71.184 kb on - strand at 71.184 kb on - strand at 71.272 kb on + strand at 71.277 kb on - strand at 71.538 kb on - strand, within GFF3802 at 71.539 kb on + strand, within GFF3802 at 71.970 kb on + strand at 72.112 kb on + strand, within GFF3803 at 72.113 kb on - strand, within GFF3803 at 72.217 kb on + strand, within GFF3803 at 72.218 kb on - strand, within GFF3803 at 72.218 kb on - strand, within GFF3803
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 69,520 - +0.1 69,632 + GFF3800 0.16 +0.2 69,740 - GFF3800 0.26 +0.4 69,749 + GFF3800 0.27 +1.8 69,813 + GFF3800 0.33 +1.3 70,045 + GFF3800 0.56 +0.1 70,046 - GFF3800 0.56 -0.2 70,123 + GFF3800 0.63 +2.2 70,308 + GFF3800 0.81 +1.0 70,309 - GFF3800 0.81 +2.0 70,309 - GFF3800 0.81 -0.4 70,309 - GFF3800 0.81 -0.4 70,309 - GFF3800 0.81 +0.4 70,312 + GFF3800 0.82 +0.3 70,312 + GFF3800 0.82 +2.0 70,501 + +0.6 70,528 + -0.2 70,621 + GFF3801 0.15 -0.5 70,622 - GFF3801 0.15 -2.3 70,760 - GFF3801 0.34 +0.2 70,765 + GFF3801 0.35 -0.8 70,766 - GFF3801 0.35 -0.0 70,794 + GFF3801 0.39 +0.6 70,835 - GFF3801 0.45 -2.5 70,959 - GFF3801 0.63 +0.1 70,981 - GFF3801 0.66 +0.7 70,999 - GFF3801 0.68 -0.9 71,080 + GFF3801 0.80 +0.3 71,184 - +0.9 71,184 - +0.2 71,272 + +0.5 71,277 - +0.1 71,538 - GFF3802 0.48 +0.0 71,539 + GFF3802 0.48 +0.4 71,970 + -0.3 72,112 + GFF3803 0.19 +1.9 72,113 - GFF3803 0.19 -1.1 72,217 + GFF3803 0.28 +0.4 72,218 - GFF3803 0.28 +0.2 72,218 - GFF3803 0.28 -1.0
Or see this region's nucleotide sequence