Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3759 and GFF3760 are separated by 106 nucleotides GFF3760 and GFF3761 are separated by 325 nucleotides
GFF3759 - ABC transporter, periplasmic substrate-binding protein, at 26,827 to 28,632
GFF3759
GFF3760 - Rtn protein, at 28,739 to 30,295
GFF3760
GFF3761 - Lipoprotein spr precursor, at 30,621 to 31,193
GFF3761
Position (kb) 28
29
30
31 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 27.820 kb on + strand, within GFF3759 at 27.882 kb on - strand, within GFF3759 at 27.965 kb on + strand, within GFF3759 at 28.090 kb on - strand, within GFF3759 at 28.090 kb on - strand, within GFF3759 at 28.167 kb on + strand, within GFF3759 at 28.169 kb on + strand, within GFF3759 at 28.169 kb on + strand, within GFF3759 at 28.369 kb on - strand, within GFF3759 at 28.399 kb on + strand, within GFF3759 at 28.424 kb on + strand, within GFF3759 at 28.446 kb on + strand, within GFF3759 at 28.446 kb on + strand, within GFF3759 at 28.447 kb on - strand, within GFF3759 at 28.530 kb on + strand at 28.824 kb on + strand at 28.825 kb on - strand at 28.992 kb on + strand, within GFF3760 at 28.997 kb on + strand, within GFF3760 at 29.107 kb on - strand, within GFF3760 at 29.188 kb on + strand, within GFF3760 at 29.322 kb on - strand, within GFF3760 at 29.490 kb on - strand, within GFF3760 at 29.523 kb on + strand, within GFF3760 at 29.523 kb on + strand, within GFF3760 at 29.524 kb on - strand, within GFF3760 at 29.528 kb on - strand, within GFF3760 at 29.617 kb on - strand, within GFF3760 at 29.647 kb on + strand, within GFF3760 at 29.773 kb on - strand, within GFF3760 at 29.778 kb on - strand, within GFF3760 at 29.778 kb on - strand, within GFF3760 at 29.778 kb on - strand, within GFF3760 at 29.782 kb on - strand, within GFF3760 at 29.924 kb on + strand, within GFF3760 at 30.138 kb on + strand, within GFF3760 at 30.163 kb on + strand at 30.212 kb on - strand at 30.218 kb on - strand at 30.348 kb on - strand at 30.463 kb on - strand at 30.591 kb on + strand at 30.623 kb on - strand at 30.693 kb on - strand, within GFF3761 at 30.693 kb on - strand, within GFF3761 at 30.743 kb on - strand, within GFF3761 at 30.743 kb on - strand, within GFF3761 at 30.743 kb on - strand, within GFF3761 at 30.798 kb on - strand, within GFF3761 at 30.845 kb on - strand, within GFF3761 at 31.086 kb on - strand, within GFF3761 at 31.226 kb on - strand at 31.261 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 27,820 + GFF3759 0.55 -2.1 27,882 - GFF3759 0.58 -0.2 27,965 + GFF3759 0.63 -0.4 28,090 - GFF3759 0.70 -2.1 28,090 - GFF3759 0.70 +1.0 28,167 + GFF3759 0.74 +0.6 28,169 + GFF3759 0.74 +0.5 28,169 + GFF3759 0.74 +0.1 28,369 - GFF3759 0.85 -0.1 28,399 + GFF3759 0.87 +0.3 28,424 + GFF3759 0.88 +0.6 28,446 + GFF3759 0.90 -0.6 28,446 + GFF3759 0.90 +0.1 28,447 - GFF3759 0.90 -0.5 28,530 + +0.4 28,824 + -0.8 28,825 - -0.3 28,992 + GFF3760 0.16 -0.5 28,997 + GFF3760 0.17 +0.1 29,107 - GFF3760 0.24 +0.2 29,188 + GFF3760 0.29 +0.1 29,322 - GFF3760 0.37 -2.1 29,490 - GFF3760 0.48 -0.2 29,523 + GFF3760 0.50 -0.4 29,523 + GFF3760 0.50 -1.4 29,524 - GFF3760 0.50 -1.1 29,528 - GFF3760 0.51 -0.5 29,617 - GFF3760 0.56 -0.1 29,647 + GFF3760 0.58 +0.1 29,773 - GFF3760 0.66 -0.8 29,778 - GFF3760 0.67 +1.2 29,778 - GFF3760 0.67 -0.5 29,778 - GFF3760 0.67 +0.1 29,782 - GFF3760 0.67 -3.9 29,924 + GFF3760 0.76 +1.7 30,138 + GFF3760 0.90 -0.7 30,163 + +0.8 30,212 - -0.1 30,218 - +2.0 30,348 - -0.4 30,463 - +0.1 30,591 + -0.0 30,623 - +0.4 30,693 - GFF3761 0.13 +0.2 30,693 - GFF3761 0.13 +0.6 30,743 - GFF3761 0.21 +0.7 30,743 - GFF3761 0.21 +0.1 30,743 - GFF3761 0.21 +1.1 30,798 - GFF3761 0.31 +0.4 30,845 - GFF3761 0.39 +0.5 31,086 - GFF3761 0.81 -0.0 31,226 - +0.4 31,261 + -1.5
Or see this region's nucleotide sequence