Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3699 and GFF3700 are separated by 334 nucleotides GFF3700 and GFF3701 are separated by 11 nucleotides GFF3701 and GFF3702 are separated by 109 nucleotides
GFF3699 - putative surface-exposed virulence protein, at 78 to 5,765
GFF3699
GFF3700 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 6,100 to 7,473
GFF3700
GFF3701 - Nitrite transporter NirC, at 7,485 to 8,294
GFF3701
GFF3702 - hypothetical protein, at 8,404 to 8,559
GFF3702
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-2
-1
0
1 at 5.177 kb on + strand, within GFF3699 at 5.178 kb on - strand, within GFF3699 at 5.180 kb on + strand, within GFF3699 at 5.236 kb on - strand at 5.236 kb on - strand at 5.236 kb on - strand at 5.236 kb on - strand at 5.318 kb on + strand at 5.489 kb on - strand at 5.489 kb on - strand at 5.498 kb on + strand at 5.499 kb on - strand at 5.601 kb on - strand at 5.601 kb on - strand at 6.416 kb on - strand, within GFF3700 at 6.709 kb on - strand, within GFF3700 at 7.140 kb on + strand, within GFF3700 at 7.141 kb on - strand, within GFF3700 at 7.321 kb on + strand, within GFF3700 at 7.486 kb on + strand at 7.596 kb on - strand, within GFF3701 at 7.644 kb on - strand, within GFF3701 at 7.645 kb on + strand, within GFF3701 at 7.646 kb on - strand, within GFF3701 at 7.749 kb on - strand, within GFF3701 at 7.927 kb on + strand, within GFF3701 at 7.985 kb on + strand, within GFF3701 at 8.127 kb on + strand, within GFF3701 at 8.185 kb on + strand, within GFF3701 at 8.186 kb on - strand, within GFF3701 at 8.186 kb on - strand, within GFF3701 at 8.273 kb on + strand at 8.366 kb on + strand at 8.366 kb on + strand at 8.374 kb on + strand at 8.374 kb on + strand at 8.375 kb on - strand at 8.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 5,177 + GFF3699 0.90 -0.1 5,178 - GFF3699 0.90 -0.3 5,180 + GFF3699 0.90 +0.4 5,236 - -0.5 5,236 - -0.1 5,236 - -1.7 5,236 - +0.1 5,318 + +0.4 5,489 - -0.0 5,489 - +0.6 5,498 + -0.1 5,499 - -0.9 5,601 - +0.1 5,601 - +0.3 6,416 - GFF3700 0.23 -1.7 6,709 - GFF3700 0.44 +0.4 7,140 + GFF3700 0.76 -2.7 7,141 - GFF3700 0.76 +0.2 7,321 + GFF3700 0.89 -2.7 7,486 + -0.2 7,596 - GFF3701 0.14 +0.0 7,644 - GFF3701 0.20 +0.2 7,645 + GFF3701 0.20 -0.4 7,646 - GFF3701 0.20 +0.6 7,749 - GFF3701 0.33 -0.4 7,927 + GFF3701 0.55 -0.5 7,985 + GFF3701 0.62 +0.2 8,127 + GFF3701 0.79 -0.1 8,185 + GFF3701 0.86 -0.1 8,186 - GFF3701 0.87 +0.2 8,186 - GFF3701 0.87 +0.7 8,273 + +0.6 8,366 + -0.4 8,366 + +0.9 8,374 + -0.0 8,374 + +0.6 8,375 - +0.6 8,375 - +0.2
Or see this region's nucleotide sequence