Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3605 and GFF3606 are separated by 11 nucleotides GFF3606 and GFF3607 are separated by 13 nucleotides GFF3607 and GFF3608 overlap by 20 nucleotides GFF3608 and GFF3609 are separated by 67 nucleotides GFF3609 and GFF3610 are separated by 149 nucleotides GFF3610 and GFF3611 are separated by 0 nucleotides
GFF3605 - Type III secretion cytoplasmic ATP synthase (EC 3.6.3.14, YscN,SpaL,MxiB,HrcN,EscN), at 357,744 to 359,045
GFF3605
GFF3606 - Type III secretion spans bacterial envelope protein (YscO), at 359,057 to 359,425
GFF3606
GFF3607 - Type III secretion protein (YscP), at 359,439 to 359,780
GFF3607
GFF3608 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 359,761 to 360,729
GFF3608
GFF3609 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 360,797 to 361,444
GFF3609
GFF3610 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 361,594 to 361,707
GFF3610
GFF3611 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 361,708 to 362,487
GFF3611
Position (kb) 359
360
361 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 358.888 kb on + strand, within GFF3605 at 358.891 kb on + strand, within GFF3605 at 358.922 kb on + strand at 358.946 kb on + strand at 359.009 kb on - strand at 359.032 kb on + strand at 359.454 kb on + strand at 359.487 kb on - strand, within GFF3607 at 359.555 kb on + strand, within GFF3607 at 359.611 kb on + strand, within GFF3607 at 359.741 kb on - strand, within GFF3607 at 359.801 kb on + strand at 359.801 kb on + strand at 359.890 kb on + strand, within GFF3608 at 359.907 kb on + strand, within GFF3608 at 359.996 kb on + strand, within GFF3608 at 359.997 kb on - strand, within GFF3608 at 359.999 kb on - strand, within GFF3608 at 360.249 kb on - strand, within GFF3608 at 360.573 kb on + strand, within GFF3608 at 360.597 kb on - strand, within GFF3608
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 358,888 + GFF3605 0.88 +0.4 358,891 + GFF3605 0.88 +0.0 358,922 + +0.1 358,946 + +0.5 359,009 - -0.5 359,032 + -0.9 359,454 + -0.3 359,487 - GFF3607 0.14 -1.1 359,555 + GFF3607 0.34 -0.1 359,611 + GFF3607 0.50 -0.2 359,741 - GFF3607 0.88 -2.6 359,801 + +0.3 359,801 + -3.3 359,890 + GFF3608 0.13 -0.5 359,907 + GFF3608 0.15 -0.4 359,996 + GFF3608 0.24 -0.8 359,997 - GFF3608 0.24 -2.2 359,999 - GFF3608 0.25 -1.0 360,249 - GFF3608 0.50 -0.9 360,573 + GFF3608 0.84 -0.9 360,597 - GFF3608 0.86 -0.8
Or see this region's nucleotide sequence