Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3548 and GFF3549 are separated by 44 nucleotides GFF3549 and GFF3550 are separated by 66 nucleotides GFF3550 and GFF3551 are separated by 363 nucleotides
GFF3548 - 3-dehydroquinate dehydratase I (EC 4.2.1.10), at 301,670 to 302,428
GFF3548
GFF3549 - Shikimate/quinate 5-dehydrogenase I beta (EC 1.1.1.282), at 302,473 to 303,339
GFF3549
GFF3550 - Putative transport system permease protein, at 303,406 to 304,641
GFF3550
GFF3551 - Putative transport system permease protein, at 305,005 to 306,216
GFF3551
Position (kb) 303
304
305 Strain fitness (log2 ratio) -2
-1
0
1 at 302.434 kb on - strand at 302.505 kb on - strand at 302.505 kb on - strand at 302.505 kb on - strand at 302.745 kb on + strand, within GFF3549 at 302.907 kb on + strand, within GFF3549 at 303.030 kb on - strand, within GFF3549 at 303.225 kb on - strand, within GFF3549 at 303.342 kb on + strand at 303.354 kb on + strand at 303.552 kb on + strand, within GFF3550 at 303.744 kb on - strand, within GFF3550 at 303.872 kb on + strand, within GFF3550 at 303.872 kb on + strand, within GFF3550 at 303.927 kb on - strand, within GFF3550 at 304.020 kb on + strand, within GFF3550 at 304.321 kb on + strand, within GFF3550 at 304.324 kb on + strand, within GFF3550 at 305.118 kb on + strand at 305.318 kb on + strand, within GFF3551 at 305.365 kb on - strand, within GFF3551 at 305.460 kb on + strand, within GFF3551 at 305.576 kb on + strand, within GFF3551 at 305.576 kb on + strand, within GFF3551
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 302,434 - +0.4 302,505 - -2.3 302,505 - +0.3 302,505 - -0.4 302,745 + GFF3549 0.31 +1.6 302,907 + GFF3549 0.50 -1.0 303,030 - GFF3549 0.64 +0.0 303,225 - GFF3549 0.87 -1.1 303,342 + -1.3 303,354 + +0.3 303,552 + GFF3550 0.12 -0.4 303,744 - GFF3550 0.27 -0.2 303,872 + GFF3550 0.38 -2.2 303,872 + GFF3550 0.38 -0.3 303,927 - GFF3550 0.42 -1.5 304,020 + GFF3550 0.50 -0.2 304,321 + GFF3550 0.74 -0.4 304,324 + GFF3550 0.74 -2.1 305,118 + -0.3 305,318 + GFF3551 0.26 -0.2 305,365 - GFF3551 0.30 -0.6 305,460 + GFF3551 0.38 -0.7 305,576 + GFF3551 0.47 -1.6 305,576 + GFF3551 0.47 -0.2
Or see this region's nucleotide sequence