Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2688 and GFF2689 overlap by 4 nucleotides GFF2689 and GFF2690 overlap by 14 nucleotides GFF2690 and GFF2691 overlap by 1 nucleotides GFF2691 and GFF2692 are separated by 1 nucleotides
GFF2688 - Peptide transport periplasmic protein SapA, at 209,410 to 210,945
GFF2688
GFF2689 - Peptide transport system permease protein SapB, at 210,942 to 211,907
GFF2689
GFF2690 - Peptide transport system permease protein SapC, at 211,894 to 212,784
GFF2690
GFF2691 - Peptide transport system ATP-binding protein SapD, at 212,784 to 213,776
GFF2691
GFF2692 - Peptide transport system ATP-binding protein SapF, at 213,778 to 214,584
GFF2692
Position (kb) 211
212
213 Strain fitness (log2 ratio) -2
-1
0
1 at 211.487 kb on + strand, within GFF2689 at 211.559 kb on + strand, within GFF2689 at 211.581 kb on + strand, within GFF2689 at 211.782 kb on + strand, within GFF2689 at 212.226 kb on - strand, within GFF2690 at 212.414 kb on + strand, within GFF2690 at 212.666 kb on + strand, within GFF2690 at 213.051 kb on - strand, within GFF2691 at 213.051 kb on - strand, within GFF2691 at 213.478 kb on + strand, within GFF2691
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 211,487 + GFF2689 0.56 -1.8 211,559 + GFF2689 0.64 -0.9 211,581 + GFF2689 0.66 -0.5 211,782 + GFF2689 0.87 -0.0 212,226 - GFF2690 0.37 -0.9 212,414 + GFF2690 0.58 -2.0 212,666 + GFF2690 0.87 -1.2 213,051 - GFF2691 0.27 -0.7 213,051 - GFF2691 0.27 -0.1 213,478 + GFF2691 0.70 -0.1
Or see this region's nucleotide sequence