Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2468 and GFF2469 are separated by 115 nucleotides GFF2469 and GFF2470 are separated by 37 nucleotides GFF2470 and GFF2471 are separated by 111 nucleotides GFF2471 and GFF2472 overlap by 1 nucleotides
GFF2468 - Putative transmembrane transport protein, at 87,899 to 89,095
GFF2468
GFF2469 - Transcriptional regulator, LysR family, at 89,211 to 90,137
GFF2469
GFF2470 - 4Fe-4S ferredoxin, iron-sulfur binding, at 90,175 to 90,435
GFF2470
GFF2471 - Holo-[acyl-carrier protein] synthase (EC 2.7.8.7), at 90,547 to 90,927
GFF2471
GFF2472 - 'Pyridoxine 5'-phosphate synthase (EC 2.6.99.2)' transl_table=11, at 90,927 to 91,658
GFF2472
Position (kb) 89
90
91 Strain fitness (log2 ratio) -1
0
1
2 at 88.251 kb on + strand, within GFF2468 at 88.983 kb on + strand at 89.484 kb on - strand, within GFF2469 at 89.801 kb on - strand, within GFF2469 at 90.082 kb on + strand at 90.083 kb on - strand at 90.136 kb on - strand at 90.214 kb on - strand, within GFF2470 at 90.510 kb on - strand at 90.528 kb on - strand at 90.548 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 88,251 + GFF2468 0.29 +0.4 88,983 + -0.6 89,484 - GFF2469 0.29 +0.2 89,801 - GFF2469 0.64 +0.9 90,082 + +0.1 90,083 - +0.0 90,136 - +0.1 90,214 - GFF2470 0.15 -0.8 90,510 - -0.1 90,528 - +2.2 90,548 + -0.4
Or see this region's nucleotide sequence