Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2140 and GFF2141 are separated by 122 nucleotides GFF2141 and GFF2142 are separated by 199 nucleotides GFF2142 and GFF2143 are separated by 7 nucleotides
GFF2140 - TldD protein, part of TldE/TldD proteolytic complex, at 31,124 to 32,569
GFF2140
GFF2141 - LysR family transcriptional regulator QseA, at 32,692 to 33,603
GFF2141
GFF2142 - probable membrane protein YPO3684, at 33,803 to 34,006
GFF2142
GFF2143 - Membrane fusion component of tripartite multidrug resistance system, at 34,014 to 34,946
GFF2143
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 31.751 kb on + strand, within GFF2140 at 31.962 kb on + strand, within GFF2140 at 31.962 kb on + strand, within GFF2140 at 31.963 kb on - strand, within GFF2140 at 32.053 kb on + strand, within GFF2140 at 32.082 kb on - strand, within GFF2140 at 32.117 kb on + strand, within GFF2140 at 32.222 kb on + strand, within GFF2140 at 32.222 kb on + strand, within GFF2140 at 32.222 kb on + strand, within GFF2140 at 32.222 kb on + strand, within GFF2140 at 32.223 kb on - strand, within GFF2140 at 32.223 kb on - strand, within GFF2140 at 32.223 kb on - strand, within GFF2140 at 32.223 kb on - strand, within GFF2140 at 32.224 kb on + strand, within GFF2140 at 32.224 kb on + strand, within GFF2140 at 32.224 kb on + strand, within GFF2140 at 32.225 kb on - strand, within GFF2140 at 32.225 kb on - strand, within GFF2140 at 32.282 kb on + strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.283 kb on - strand, within GFF2140 at 32.354 kb on + strand, within GFF2140 at 32.458 kb on + strand at 32.910 kb on - strand, within GFF2141 at 32.961 kb on + strand, within GFF2141 at 33.046 kb on - strand, within GFF2141 at 33.047 kb on + strand, within GFF2141 at 33.345 kb on + strand, within GFF2141 at 33.345 kb on + strand, within GFF2141 at 33.346 kb on - strand, within GFF2141 at 33.565 kb on - strand at 34.053 kb on - strand at 34.107 kb on + strand at 34.107 kb on + strand at 34.161 kb on - strand, within GFF2143 at 34.195 kb on + strand, within GFF2143 at 34.195 kb on + strand, within GFF2143 at 34.196 kb on - strand, within GFF2143 at 34.294 kb on + strand, within GFF2143 at 34.295 kb on - strand, within GFF2143 at 34.303 kb on - strand, within GFF2143 at 34.310 kb on - strand, within GFF2143 at 34.481 kb on + strand, within GFF2143 at 34.599 kb on + strand, within GFF2143 at 34.599 kb on + strand, within GFF2143
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 31,751 + GFF2140 0.43 -2.1 31,962 + GFF2140 0.58 -0.5 31,962 + GFF2140 0.58 -1.0 31,963 - GFF2140 0.58 -2.3 32,053 + GFF2140 0.64 +0.0 32,082 - GFF2140 0.66 -0.5 32,117 + GFF2140 0.69 -0.9 32,222 + GFF2140 0.76 -1.0 32,222 + GFF2140 0.76 +0.1 32,222 + GFF2140 0.76 -0.6 32,222 + GFF2140 0.76 +0.6 32,223 - GFF2140 0.76 +0.3 32,223 - GFF2140 0.76 -3.2 32,223 - GFF2140 0.76 -0.7 32,223 - GFF2140 0.76 -0.4 32,224 + GFF2140 0.76 +0.2 32,224 + GFF2140 0.76 +0.6 32,224 + GFF2140 0.76 +0.0 32,225 - GFF2140 0.76 +0.1 32,225 - GFF2140 0.76 -0.2 32,282 + GFF2140 0.80 -1.3 32,283 - GFF2140 0.80 -1.0 32,283 - GFF2140 0.80 -0.1 32,283 - GFF2140 0.80 +0.5 32,283 - GFF2140 0.80 -0.2 32,283 - GFF2140 0.80 -0.1 32,283 - GFF2140 0.80 +0.9 32,354 + GFF2140 0.85 -1.0 32,458 + +0.2 32,910 - GFF2141 0.24 -0.2 32,961 + GFF2141 0.29 +0.2 33,046 - GFF2141 0.39 -0.0 33,047 + GFF2141 0.39 +0.1 33,345 + GFF2141 0.72 +1.5 33,345 + GFF2141 0.72 -1.3 33,346 - GFF2141 0.72 -0.2 33,565 - -0.2 34,053 - -0.0 34,107 + -0.0 34,107 + -0.5 34,161 - GFF2143 0.16 +0.3 34,195 + GFF2143 0.19 +0.2 34,195 + GFF2143 0.19 +0.5 34,196 - GFF2143 0.20 +0.0 34,294 + GFF2143 0.30 -0.1 34,295 - GFF2143 0.30 -0.6 34,303 - GFF2143 0.31 -0.3 34,310 - GFF2143 0.32 +0.6 34,481 + GFF2143 0.50 -3.2 34,599 + GFF2143 0.63 -0.3 34,599 + GFF2143 0.63 -0.3
Or see this region's nucleotide sequence