Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1918 and GFF1919 are separated by 237 nucleotides GFF1919 and GFF1920 are separated by 77 nucleotides GFF1920 and GFF1921 are separated by 88 nucleotides GFF1921 and GFF1922 are separated by 26 nucleotides
GFF1918 - Histidine ABC transporter, histidine-binding periplasmic protein precursor HisJ (TC 3.A.1.3.1), at 110,881 to 111,663
GFF1918
GFF1919 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 111,901 to 112,683
GFF1919
GFF1920 - hypothetical protein, at 112,761 to 112,883
GFF1920
GFF1921 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-), at 112,972 to 113,541
GFF1921
GFF1922 - Lysine-specific permease, at 113,568 to 114,974
GFF1922
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-2
-1
0
1 at 110.935 kb on - strand at 111.010 kb on - strand, within GFF1918 at 111.063 kb on + strand, within GFF1918 at 111.066 kb on - strand, within GFF1918 at 111.159 kb on - strand, within GFF1918 at 111.348 kb on + strand, within GFF1918 at 111.462 kb on + strand, within GFF1918 at 111.462 kb on + strand, within GFF1918 at 111.462 kb on + strand, within GFF1918 at 111.463 kb on - strand, within GFF1918 at 111.463 kb on - strand, within GFF1918 at 111.478 kb on - strand, within GFF1918 at 111.557 kb on + strand, within GFF1918 at 111.557 kb on + strand, within GFF1918 at 111.557 kb on + strand, within GFF1918 at 111.558 kb on - strand, within GFF1918 at 111.561 kb on + strand, within GFF1918 at 111.728 kb on + strand at 112.056 kb on + strand, within GFF1919 at 112.179 kb on - strand, within GFF1919 at 112.200 kb on - strand, within GFF1919 at 112.452 kb on + strand, within GFF1919 at 112.497 kb on + strand, within GFF1919 at 112.498 kb on - strand, within GFF1919 at 112.539 kb on + strand, within GFF1919 at 112.577 kb on + strand, within GFF1919 at 112.584 kb on + strand, within GFF1919 at 112.584 kb on + strand, within GFF1919 at 112.584 kb on + strand, within GFF1919 at 112.585 kb on - strand, within GFF1919 at 112.585 kb on - strand, within GFF1919 at 112.841 kb on - strand, within GFF1920 at 112.949 kb on + strand at 113.119 kb on + strand, within GFF1921 at 113.244 kb on + strand, within GFF1921 at 113.245 kb on - strand, within GFF1921 at 113.482 kb on + strand, within GFF1921 at 113.496 kb on - strand at 113.524 kb on + strand at 113.640 kb on - strand at 113.661 kb on + strand at 113.668 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 110,935 - +1.0 111,010 - GFF1918 0.16 +0.2 111,063 + GFF1918 0.23 +0.5 111,066 - GFF1918 0.24 +0.3 111,159 - GFF1918 0.36 -0.1 111,348 + GFF1918 0.60 -0.7 111,462 + GFF1918 0.74 +0.1 111,462 + GFF1918 0.74 -0.4 111,462 + GFF1918 0.74 -0.9 111,463 - GFF1918 0.74 -0.3 111,463 - GFF1918 0.74 +0.3 111,478 - GFF1918 0.76 +0.2 111,557 + GFF1918 0.86 -0.4 111,557 + GFF1918 0.86 +0.2 111,557 + GFF1918 0.86 -0.8 111,558 - GFF1918 0.86 +0.0 111,561 + GFF1918 0.87 -0.4 111,728 + +0.3 112,056 + GFF1919 0.20 +1.3 112,179 - GFF1919 0.36 +0.8 112,200 - GFF1919 0.38 +0.2 112,452 + GFF1919 0.70 -1.3 112,497 + GFF1919 0.76 +0.7 112,498 - GFF1919 0.76 +1.0 112,539 + GFF1919 0.81 -0.0 112,577 + GFF1919 0.86 -0.1 112,584 + GFF1919 0.87 -0.4 112,584 + GFF1919 0.87 +0.0 112,584 + GFF1919 0.87 +0.0 112,585 - GFF1919 0.87 +1.2 112,585 - GFF1919 0.87 -0.4 112,841 - GFF1920 0.65 +0.3 112,949 + +1.0 113,119 + GFF1921 0.26 +1.5 113,244 + GFF1921 0.48 -2.6 113,245 - GFF1921 0.48 +0.5 113,482 + GFF1921 0.89 -0.6 113,496 - +0.3 113,524 + -0.5 113,640 - +0.8 113,661 + +1.6 113,668 - +0.8
Or see this region's nucleotide sequence