Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1803 and GFF1804 are separated by 110 nucleotides GFF1804 and GFF1805 are separated by 75 nucleotides
GFF1803 - Exoenzymes regulatory protein AepA precursor, at 24,267 to 25,967
GFF1803
GFF1804 - Sensory histidine kinase in two-component regulatory system with RstA, at 26,078 to 27,379
GFF1804
GFF1805 - DNA replication terminus site-binding protein, at 27,455 to 28,384
GFF1805
Position (kb) 26
27
28 Strain fitness (log2 ratio) -2
-1
0
1 at 25.363 kb on - strand, within GFF1803 at 25.408 kb on + strand, within GFF1803 at 25.408 kb on + strand, within GFF1803 at 25.509 kb on + strand, within GFF1803 at 25.510 kb on - strand, within GFF1803 at 25.510 kb on - strand, within GFF1803 at 25.699 kb on + strand, within GFF1803 at 25.700 kb on - strand, within GFF1803 at 25.770 kb on - strand, within GFF1803 at 25.865 kb on + strand at 25.865 kb on + strand at 26.051 kb on + strand at 26.219 kb on + strand, within GFF1804 at 26.323 kb on - strand, within GFF1804 at 26.332 kb on + strand, within GFF1804 at 26.332 kb on + strand, within GFF1804 at 26.396 kb on + strand, within GFF1804 at 26.404 kb on + strand, within GFF1804 at 26.405 kb on - strand, within GFF1804 at 26.499 kb on + strand, within GFF1804 at 26.692 kb on + strand, within GFF1804 at 26.830 kb on + strand, within GFF1804 at 27.000 kb on + strand, within GFF1804 at 27.065 kb on - strand, within GFF1804 at 27.085 kb on + strand, within GFF1804 at 27.184 kb on - strand, within GFF1804 at 27.265 kb on + strand at 27.417 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.419 kb on + strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.420 kb on - strand at 27.451 kb on + strand at 27.451 kb on + strand at 27.533 kb on - strand at 27.557 kb on - strand, within GFF1805 at 27.619 kb on + strand, within GFF1805 at 27.777 kb on + strand, within GFF1805 at 27.895 kb on - strand, within GFF1805 at 27.937 kb on + strand, within GFF1805 at 28.184 kb on + strand, within GFF1805 at 28.185 kb on - strand, within GFF1805 at 28.239 kb on + strand, within GFF1805 at 28.307 kb on - strand at 28.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 25,363 - GFF1803 0.64 -0.9 25,408 + GFF1803 0.67 +1.7 25,408 + GFF1803 0.67 +1.2 25,509 + GFF1803 0.73 +0.3 25,510 - GFF1803 0.73 -1.2 25,510 - GFF1803 0.73 -0.3 25,699 + GFF1803 0.84 +0.4 25,700 - GFF1803 0.84 -0.7 25,770 - GFF1803 0.88 +0.9 25,865 + -0.2 25,865 + -1.1 26,051 + -0.0 26,219 + GFF1804 0.11 -2.5 26,323 - GFF1804 0.19 -1.1 26,332 + GFF1804 0.20 -0.5 26,332 + GFF1804 0.20 -2.1 26,396 + GFF1804 0.24 -1.5 26,404 + GFF1804 0.25 -0.1 26,405 - GFF1804 0.25 -0.4 26,499 + GFF1804 0.32 -2.0 26,692 + GFF1804 0.47 -1.1 26,830 + GFF1804 0.58 +0.1 27,000 + GFF1804 0.71 -0.2 27,065 - GFF1804 0.76 -0.8 27,085 + GFF1804 0.77 +0.1 27,184 - GFF1804 0.85 -2.6 27,265 + -0.4 27,417 + +0.3 27,419 + +0.1 27,419 + +0.1 27,419 + +0.5 27,419 + +0.6 27,419 + +1.6 27,419 + +0.3 27,420 - -0.8 27,420 - -1.7 27,420 - +1.5 27,451 + +1.1 27,451 + +0.8 27,533 - +0.0 27,557 - GFF1805 0.11 -2.6 27,619 + GFF1805 0.18 +1.4 27,777 + GFF1805 0.35 +0.3 27,895 - GFF1805 0.47 -0.5 27,937 + GFF1805 0.52 +0.4 28,184 + GFF1805 0.78 -0.4 28,185 - GFF1805 0.78 -0.6 28,239 + GFF1805 0.84 +1.4 28,307 - -0.5 28,371 - +1.2
Or see this region's nucleotide sequence