Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1696 and GFF1697 are separated by 66 nucleotides GFF1697 and GFF1698 overlap by 4 nucleotides GFF1698 and GFF1699 are separated by 311 nucleotides GFF1699 and GFF1700 overlap by 4 nucleotides
GFF1696 - N-3-oxohexanoyl-L-homoserine lactone quorum-sensing transcriptional activator @ N-3-oxooctanoyl-L-homoserine lactone quorum-sensing transcriptional activator, at 5,301 to 6,023
GFF1696
GFF1697 - FIG00634007: hypothetical protein, at 6,090 to 6,314
GFF1697
GFF1698 - FIG01045369: hypothetical protein, at 6,311 to 6,466
GFF1698
GFF1699 - BarA-associated response regulator UvrY (= GacA = SirA), at 6,778 to 7,434
GFF1699
GFF1700 - Excinuclease ABC subunit C, at 7,431 to 9,263
GFF1700
Position (kb) 6
7
8 Strain fitness (log2 ratio) -2
-1
0
1
2 at 6.186 kb on - strand, within GFF1697 at 6.652 kb on - strand at 6.652 kb on - strand at 6.742 kb on + strand at 6.847 kb on - strand, within GFF1699 at 6.913 kb on - strand, within GFF1699 at 7.147 kb on + strand, within GFF1699 at 7.311 kb on + strand, within GFF1699 at 7.422 kb on - strand at 7.496 kb on + strand at 7.497 kb on - strand at 7.501 kb on - strand at 7.680 kb on + strand, within GFF1700 at 7.704 kb on + strand, within GFF1700 at 7.728 kb on - strand, within GFF1700 at 7.742 kb on + strand, within GFF1700 at 7.829 kb on + strand, within GFF1700 at 7.903 kb on + strand, within GFF1700 at 8.010 kb on + strand, within GFF1700 at 8.010 kb on + strand, within GFF1700 at 8.033 kb on + strand, within GFF1700 at 8.114 kb on + strand, within GFF1700 at 8.381 kb on - strand, within GFF1700
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 6,186 - GFF1697 0.43 -0.4 6,652 - +0.4 6,652 - +0.1 6,742 + -0.0 6,847 - GFF1699 0.11 +2.0 6,913 - GFF1699 0.21 -0.3 7,147 + GFF1699 0.56 +0.4 7,311 + GFF1699 0.81 +1.7 7,422 - -0.0 7,496 + -0.9 7,497 - -0.9 7,501 - -0.3 7,680 + GFF1700 0.14 -0.5 7,704 + GFF1700 0.15 +0.8 7,728 - GFF1700 0.16 -1.8 7,742 + GFF1700 0.17 -0.1 7,829 + GFF1700 0.22 -0.1 7,903 + GFF1700 0.26 +0.0 8,010 + GFF1700 0.32 -0.7 8,010 + GFF1700 0.32 -0.7 8,033 + GFF1700 0.33 +0.2 8,114 + GFF1700 0.37 -0.6 8,381 - GFF1700 0.52 -1.0
Or see this region's nucleotide sequence