Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1676 and GFF1677 are separated by 632 nucleotides GFF1677 and GFF1678 are separated by 550 nucleotides
GFF1676 - Trehalose-6-phosphate phosphatase (EC 3.1.3.12), at 61,117 to 61,920
GFF1676
GFF1677 - DJ-1/YajL/PfpI superfamily, includes chaperone protein YajL (former ThiJ), parkinsonism-associated protein DJ-1, peptidases PfpI, Hsp31, at 62,553 to 63,110
GFF1677
GFF1678 - Ferritin-like protein 2, at 63,661 to 64,164
GFF1678
Position (kb) 62
63
64 Strain fitness (log2 ratio) -1
0
1 at 61.576 kb on + strand, within GFF1676 at 61.592 kb on - strand, within GFF1676 at 61.648 kb on - strand, within GFF1676 at 61.787 kb on - strand, within GFF1676 at 61.976 kb on + strand at 61.976 kb on + strand at 61.981 kb on - strand at 62.008 kb on + strand at 62.109 kb on + strand at 62.111 kb on + strand at 62.222 kb on - strand at 62.346 kb on - strand at 62.436 kb on - strand at 62.524 kb on + strand at 62.566 kb on + strand at 62.567 kb on - strand at 62.571 kb on - strand at 62.571 kb on - strand at 62.704 kb on + strand, within GFF1677 at 62.750 kb on - strand, within GFF1677 at 62.904 kb on + strand, within GFF1677 at 62.969 kb on + strand, within GFF1677 at 62.970 kb on - strand, within GFF1677 at 63.053 kb on + strand, within GFF1677 at 63.054 kb on - strand, within GFF1677 at 63.172 kb on - strand at 63.561 kb on + strand at 63.731 kb on - strand, within GFF1678 at 63.741 kb on - strand, within GFF1678 at 64.092 kb on + strand, within GFF1678
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 61,576 + GFF1676 0.57 -1.3 61,592 - GFF1676 0.59 +0.7 61,648 - GFF1676 0.66 +0.6 61,787 - GFF1676 0.83 -0.5 61,976 + +0.2 61,976 + +0.3 61,981 - -0.5 62,008 + -0.5 62,109 + -0.1 62,111 + +1.5 62,222 - -0.6 62,346 - -0.1 62,436 - +0.2 62,524 + +0.1 62,566 + -0.3 62,567 - +0.2 62,571 - -0.3 62,571 - -0.6 62,704 + GFF1677 0.27 +1.5 62,750 - GFF1677 0.35 -1.2 62,904 + GFF1677 0.63 -0.1 62,969 + GFF1677 0.75 -0.1 62,970 - GFF1677 0.75 +0.5 63,053 + GFF1677 0.90 -0.1 63,054 - GFF1677 0.90 +0.3 63,172 - +0.8 63,561 + +0.8 63,731 - GFF1678 0.14 -0.1 63,741 - GFF1678 0.16 -0.4 64,092 + GFF1678 0.86 +1.1
Or see this region's nucleotide sequence