Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF550 and GFF551 overlap by 4 nucleotides GFF551 and GFF552 are separated by 1 nucleotides GFF552 and GFF553 are separated by 131 nucleotides
GFF550 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 281,668 to 282,945
GFF550
GFF551 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 282,942 to 283,709
GFF551
GFF552 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 283,711 to 284,424
GFF552
GFF553 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 284,556 to 284,777
GFF553
Position (kb) 282
283
284 Strain fitness (log2 ratio) -2
-1
0
1 at 282.029 kb on - strand, within GFF550 at 282.118 kb on + strand, within GFF550 at 282.118 kb on + strand, within GFF550 at 282.120 kb on + strand, within GFF550 at 282.146 kb on - strand, within GFF550 at 282.146 kb on - strand, within GFF550 at 282.286 kb on - strand, within GFF550 at 282.579 kb on - strand, within GFF550 at 282.616 kb on - strand, within GFF550 at 282.955 kb on + strand at 283.085 kb on + strand, within GFF551 at 283.104 kb on + strand, within GFF551 at 283.541 kb on + strand, within GFF551 at 283.580 kb on + strand, within GFF551 at 283.693 kb on - strand at 283.708 kb on - strand at 283.708 kb on - strand at 283.908 kb on + strand, within GFF552 at 283.917 kb on - strand, within GFF552 at 284.059 kb on - strand, within GFF552 at 284.072 kb on - strand, within GFF552 at 284.183 kb on + strand, within GFF552 at 284.184 kb on - strand, within GFF552 at 284.410 kb on + strand at 284.411 kb on - strand at 284.530 kb on - strand at 284.538 kb on - strand at 284.571 kb on + strand at 284.572 kb on - strand at 284.572 kb on - strand at 284.572 kb on - strand at 284.654 kb on + strand, within GFF553
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 282,029 - GFF550 0.28 -0.4 282,118 + GFF550 0.35 +0.2 282,118 + GFF550 0.35 +0.8 282,120 + GFF550 0.35 -0.4 282,146 - GFF550 0.37 -0.2 282,146 - GFF550 0.37 +0.8 282,286 - GFF550 0.48 +0.3 282,579 - GFF550 0.71 +0.1 282,616 - GFF550 0.74 -0.1 282,955 + -2.2 283,085 + GFF551 0.19 +0.5 283,104 + GFF551 0.21 +0.1 283,541 + GFF551 0.78 +0.9 283,580 + GFF551 0.83 -0.2 283,693 - +0.7 283,708 - -0.5 283,708 - +0.2 283,908 + GFF552 0.28 +0.7 283,917 - GFF552 0.29 -0.3 284,059 - GFF552 0.49 -1.2 284,072 - GFF552 0.51 -0.3 284,183 + GFF552 0.66 -0.6 284,184 - GFF552 0.66 -0.4 284,410 + +0.0 284,411 - +0.1 284,530 - -0.4 284,538 - +0.6 284,571 + -0.5 284,572 - -0.4 284,572 - +1.5 284,572 - +0.1 284,654 + GFF553 0.44 +0.1
Or see this region's nucleotide sequence