Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4865 and GFF4866 are separated by 346 nucleotides GFF4866 and GFF4867 are separated by 192 nucleotides GFF4867 and GFF4868 overlap by 1 nucleotides GFF4868 and GFF4869 are separated by 272 nucleotides
GFF4865 - Glycine cleavage system transcriptional activator GcvA, at 164,237 to 165,154
GFF4865
GFF4866 - probable lipoprotein, at 165,501 to 165,728
GFF4866
GFF4867 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 165,921 to 167,126
GFF4867
GFF4868 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 167,126 to 167,569
GFF4868
GFF4869 - FIG00761799: membrane protein, at 167,842 to 168,756
GFF4869
Position (kb) 165
166
167
168 Strain fitness (log2 ratio) -1
0
1 at 164.936 kb on - strand, within GFF4865 at 165.656 kb on - strand, within GFF4866 at 165.696 kb on - strand, within GFF4866 at 165.755 kb on + strand at 165.820 kb on - strand at 165.823 kb on + strand at 165.838 kb on + strand at 165.838 kb on + strand at 165.857 kb on + strand at 165.871 kb on + strand at 166.113 kb on + strand, within GFF4867 at 166.147 kb on + strand, within GFF4867 at 166.256 kb on + strand, within GFF4867 at 166.435 kb on + strand, within GFF4867 at 166.591 kb on - strand, within GFF4867 at 166.963 kb on - strand, within GFF4867 at 167.057 kb on + strand at 167.057 kb on + strand at 167.124 kb on + strand at 167.141 kb on + strand at 167.488 kb on - strand, within GFF4868 at 167.582 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 164,936 - GFF4865 0.76 +0.2 165,656 - GFF4866 0.68 +0.2 165,696 - GFF4866 0.86 +0.6 165,755 + +0.7 165,820 - -0.3 165,823 + +0.2 165,838 + +0.2 165,838 + +0.2 165,857 + +0.1 165,871 + +0.2 166,113 + GFF4867 0.16 +0.2 166,147 + GFF4867 0.19 -0.1 166,256 + GFF4867 0.28 -0.2 166,435 + GFF4867 0.43 -1.2 166,591 - GFF4867 0.56 -0.8 166,963 - GFF4867 0.86 -0.6 167,057 + +0.4 167,057 + +0.1 167,124 + +0.8 167,141 + +0.2 167,488 - GFF4868 0.82 -0.9 167,582 - -0.3
Or see this region's nucleotide sequence