Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4501 and GFF4502 are separated by 13 nucleotides GFF4502 and GFF4503 overlap by 1 nucleotides GFF4503 and GFF4504 are separated by 2 nucleotides
GFF4501 - 2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) [enterobactin] siderophore, at 287,654 to 289,264
GFF4501
GFF4502 - Isochorismatase (EC 3.3.2.1) of siderophore biosynthesis, at 289,278 to 290,135
GFF4502
GFF4503 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EC 1.3.1.28) of siderophore biosynthesis, at 290,135 to 290,890
GFF4503
GFF4504 - Proofreading thioesterase in enterobactin biosynthesis EntH, at 290,893 to 291,306
GFF4504
Position (kb) 289
290
291 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 288.278 kb on + strand, within GFF4501 at 288.356 kb on - strand, within GFF4501 at 288.359 kb on + strand, within GFF4501 at 288.360 kb on - strand, within GFF4501 at 288.360 kb on - strand, within GFF4501 at 288.514 kb on - strand, within GFF4501 at 288.994 kb on - strand, within GFF4501 at 289.083 kb on - strand, within GFF4501 at 289.093 kb on - strand, within GFF4501 at 289.093 kb on - strand, within GFF4501 at 289.120 kb on - strand at 289.302 kb on + strand at 289.388 kb on + strand, within GFF4502 at 289.389 kb on - strand, within GFF4502 at 289.478 kb on + strand, within GFF4502 at 289.479 kb on - strand, within GFF4502 at 289.503 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.557 kb on - strand, within GFF4502 at 289.604 kb on - strand, within GFF4502 at 289.654 kb on - strand, within GFF4502 at 289.739 kb on + strand, within GFF4502 at 289.852 kb on - strand, within GFF4502 at 289.852 kb on - strand, within GFF4502 at 290.015 kb on - strand, within GFF4502 at 290.051 kb on - strand at 290.133 kb on + strand at 290.386 kb on + strand, within GFF4503 at 290.548 kb on + strand, within GFF4503 at 290.563 kb on + strand, within GFF4503 at 290.584 kb on + strand, within GFF4503 at 290.651 kb on - strand, within GFF4503 at 290.842 kb on + strand at 290.842 kb on + strand at 290.843 kb on - strand at 290.974 kb on + strand, within GFF4504 at 290.975 kb on - strand, within GFF4504 at 291.103 kb on + strand, within GFF4504
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 288,278 + GFF4501 0.39 +0.4 288,356 - GFF4501 0.44 +0.2 288,359 + GFF4501 0.44 +0.4 288,360 - GFF4501 0.44 +0.3 288,360 - GFF4501 0.44 -0.3 288,514 - GFF4501 0.53 +0.5 288,994 - GFF4501 0.83 -0.2 289,083 - GFF4501 0.89 -1.2 289,093 - GFF4501 0.89 +0.5 289,093 - GFF4501 0.89 +0.6 289,120 - -0.9 289,302 + -0.8 289,388 + GFF4502 0.13 -0.3 289,389 - GFF4502 0.13 +0.6 289,478 + GFF4502 0.23 -0.1 289,479 - GFF4502 0.23 +0.4 289,503 + GFF4502 0.26 +0.4 289,556 + GFF4502 0.32 -0.7 289,556 + GFF4502 0.32 +0.4 289,556 + GFF4502 0.32 +0.1 289,557 - GFF4502 0.33 -1.0 289,604 - GFF4502 0.38 -3.1 289,654 - GFF4502 0.44 -0.1 289,739 + GFF4502 0.54 +0.4 289,852 - GFF4502 0.67 -0.1 289,852 - GFF4502 0.67 +0.1 290,015 - GFF4502 0.86 -0.5 290,051 - +0.7 290,133 + +0.1 290,386 + GFF4503 0.33 -0.4 290,548 + GFF4503 0.55 -0.7 290,563 + GFF4503 0.57 -0.6 290,584 + GFF4503 0.59 -0.8 290,651 - GFF4503 0.68 -0.1 290,842 + +0.3 290,842 + -0.4 290,843 - -1.3 290,974 + GFF4504 0.20 -0.3 290,975 - GFF4504 0.20 -0.7 291,103 + GFF4504 0.51 +0.8
Or see this region's nucleotide sequence