Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4279 and GFF4280 are separated by 23 nucleotides GFF4280 and GFF4281 are separated by 166 nucleotides GFF4281 and GFF4282 are separated by 91 nucleotides GFF4282 and GFF4283 are separated by 14 nucleotides GFF4283 and GFF4284 are separated by 33 nucleotides
GFF4279 - protein iraP, at 67,317 to 67,577
GFF4279
GFF4280 - FIG01046247: hypothetical protein, at 67,601 to 67,762
GFF4280
GFF4281 - Phosphate starvation-inducible protein PsiF, at 67,929 to 68,249
GFF4281
GFF4282 - Protein YaiC, at 68,341 to 69,453
GFF4282
GFF4283 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 69,468 to 70,277
GFF4283
GFF4284 - hypothetical protein, at 70,311 to 70,460
GFF4284
GFF4285 - Protein YaiI, at 70,417 to 70,872
GFF4285
Position (kb)
68
69
70 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 67.393 kb on + strand, within GFF4279 at 67.426 kb on + strand, within GFF4279 at 67.431 kb on - strand, within GFF4279 at 67.471 kb on + strand, within GFF4279 at 67.582 kb on - strand at 67.859 kb on + strand at 67.860 kb on - strand at 67.899 kb on + strand at 67.943 kb on - strand at 68.247 kb on + strand at 68.293 kb on - strand at 68.317 kb on - strand at 68.694 kb on + strand, within GFF4282 at 68.694 kb on + strand, within GFF4282 at 68.695 kb on - strand, within GFF4282 at 68.881 kb on - strand, within GFF4282 at 68.909 kb on + strand, within GFF4282 at 68.943 kb on + strand, within GFF4282 at 68.943 kb on + strand, within GFF4282 at 68.993 kb on - strand, within GFF4282 at 69.024 kb on + strand, within GFF4282 at 69.173 kb on + strand, within GFF4282 at 69.225 kb on + strand, within GFF4282 at 69.226 kb on - strand, within GFF4282 at 69.255 kb on + strand, within GFF4282 at 69.293 kb on + strand, within GFF4282 at 69.323 kb on + strand, within GFF4282 at 69.323 kb on + strand, within GFF4282 at 69.324 kb on - strand, within GFF4282 at 69.324 kb on - strand, within GFF4282 at 69.406 kb on + strand at 69.407 kb on - strand at 69.429 kb on + strand at 69.519 kb on + strand at 69.520 kb on - strand at 69.597 kb on - strand, within GFF4283 at 69.675 kb on - strand, within GFF4283 at 69.676 kb on + strand, within GFF4283 at 69.737 kb on + strand, within GFF4283 at 69.738 kb on - strand, within GFF4283 at 69.738 kb on - strand, within GFF4283 at 69.745 kb on + strand, within GFF4283 at 69.745 kb on + strand, within GFF4283 at 70.052 kb on + strand, within GFF4283 at 70.174 kb on + strand, within GFF4283 at 70.175 kb on - strand, within GFF4283 at 70.175 kb on - strand, within GFF4283 at 70.197 kb on + strand at 70.241 kb on + strand at 70.257 kb on - strand at 70.397 kb on + strand, within GFF4284 at 70.399 kb on + strand, within GFF4284 at 70.405 kb on - strand, within GFF4284 at 70.417 kb on - strand at 70.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 67,393 + GFF4279 0.29 -0.6 67,426 + GFF4279 0.42 -3.3 67,431 - GFF4279 0.44 -0.8 67,471 + GFF4279 0.59 +0.7 67,582 - +0.7 67,859 + -0.3 67,860 - -0.7 67,899 + -0.6 67,943 - +0.8 68,247 + +0.3 68,293 - -0.6 68,317 - +0.5 68,694 + GFF4282 0.32 -0.0 68,694 + GFF4282 0.32 +0.3 68,695 - GFF4282 0.32 +0.2 68,881 - GFF4282 0.49 -0.0 68,909 + GFF4282 0.51 +0.3 68,943 + GFF4282 0.54 +0.7 68,943 + GFF4282 0.54 +1.4 68,993 - GFF4282 0.59 +0.3 69,024 + GFF4282 0.61 +0.4 69,173 + GFF4282 0.75 +0.4 69,225 + GFF4282 0.79 +0.4 69,226 - GFF4282 0.80 -0.2 69,255 + GFF4282 0.82 -0.5 69,293 + GFF4282 0.86 +0.4 69,323 + GFF4282 0.88 +0.3 69,323 + GFF4282 0.88 -0.8 69,324 - GFF4282 0.88 +1.1 69,324 - GFF4282 0.88 +0.1 69,406 + +0.4 69,407 - -2.9 69,429 + +0.0 69,519 + -1.0 69,520 - +0.7 69,597 - GFF4283 0.16 +0.0 69,675 - GFF4283 0.26 -0.6 69,676 + GFF4283 0.26 -0.1 69,737 + GFF4283 0.33 -1.0 69,738 - GFF4283 0.33 +0.2 69,738 - GFF4283 0.33 -1.4 69,745 + GFF4283 0.34 -0.2 69,745 + GFF4283 0.34 -0.8 70,052 + GFF4283 0.72 -0.6 70,174 + GFF4283 0.87 +0.3 70,175 - GFF4283 0.87 +0.6 70,175 - GFF4283 0.87 +0.5 70,197 + +0.6 70,241 + -0.5 70,257 - +0.1 70,397 + GFF4284 0.57 -0.3 70,399 + GFF4284 0.59 -0.7 70,405 - GFF4284 0.63 +0.2 70,417 - -3.4 70,417 - +0.7
Or see this region's nucleotide sequence