Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF421 and GFF422 are separated by 284 nucleotides GFF422 and GFF423 are separated by 168 nucleotides
GFF421 - Selenocysteine-specific translation elongation factor, at 140,382 to 142,232
GFF421
GFF422 - Putative sugar kinase, at 142,517 to 143,398
GFF422
GFF423 - Aldehyde dehydrogenase B (EC 1.2.1.22), at 143,567 to 145,105
GFF423
Position (kb)
142
143
144 Strain fitness (log2 ratio)
-2
-1
0
1 at 141.552 kb on - strand, within GFF421 at 141.659 kb on + strand, within GFF421 at 141.672 kb on + strand, within GFF421 at 141.801 kb on - strand, within GFF421 at 141.833 kb on + strand, within GFF421 at 142.014 kb on + strand, within GFF421 at 142.214 kb on - strand at 142.342 kb on + strand at 142.395 kb on - strand at 142.443 kb on + strand at 142.728 kb on + strand, within GFF422 at 142.869 kb on - strand, within GFF422 at 142.891 kb on + strand, within GFF422 at 142.892 kb on - strand, within GFF422 at 142.898 kb on - strand, within GFF422 at 142.982 kb on - strand, within GFF422 at 143.342 kb on - strand at 143.389 kb on - strand at 143.398 kb on + strand at 143.398 kb on + strand at 143.399 kb on - strand at 143.431 kb on + strand at 143.432 kb on - strand at 143.435 kb on - strand at 143.471 kb on - strand at 143.543 kb on + strand at 143.544 kb on - strand at 143.554 kb on + strand at 143.628 kb on + strand at 143.650 kb on + strand at 143.783 kb on - strand, within GFF423 at 143.806 kb on + strand, within GFF423 at 143.814 kb on + strand, within GFF423 at 143.938 kb on - strand, within GFF423 at 143.977 kb on + strand, within GFF423 at 143.978 kb on - strand, within GFF423 at 143.978 kb on - strand, within GFF423 at 143.978 kb on - strand, within GFF423 at 143.980 kb on - strand, within GFF423 at 144.336 kb on - strand, within GFF423 at 144.392 kb on - strand, within GFF423 at 144.392 kb on - strand, within GFF423
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 141,552 - GFF421 0.63 +0.5 141,659 + GFF421 0.69 -0.6 141,672 + GFF421 0.70 +1.6 141,801 - GFF421 0.77 -0.3 141,833 + GFF421 0.78 -0.4 142,014 + GFF421 0.88 -0.4 142,214 - -0.2 142,342 + -0.8 142,395 - +0.8 142,443 + +0.7 142,728 + GFF422 0.24 -0.1 142,869 - GFF422 0.40 +0.0 142,891 + GFF422 0.42 -0.2 142,892 - GFF422 0.43 +0.2 142,898 - GFF422 0.43 -0.2 142,982 - GFF422 0.53 +1.1 143,342 - -0.5 143,389 - +0.2 143,398 + -1.9 143,398 + +0.4 143,399 - -2.0 143,431 + -0.1 143,432 - +0.1 143,435 - +0.2 143,471 - -0.5 143,543 + -0.2 143,544 - -0.3 143,554 + +0.1 143,628 + +0.1 143,650 + -0.2 143,783 - GFF423 0.14 +0.1 143,806 + GFF423 0.16 +0.4 143,814 + GFF423 0.16 +0.5 143,938 - GFF423 0.24 -1.2 143,977 + GFF423 0.27 +1.2 143,978 - GFF423 0.27 -0.2 143,978 - GFF423 0.27 +0.6 143,978 - GFF423 0.27 -1.3 143,980 - GFF423 0.27 -0.5 144,336 - GFF423 0.50 +0.2 144,392 - GFF423 0.54 +1.0 144,392 - GFF423 0.54 +0.9
Or see this region's nucleotide sequence