Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF395 and GFF396 overlap by 14 nucleotides GFF396 and GFF397 are separated by 3 nucleotides
GFF395 - Beta-1,3-galactosyltransferase / Beta-1,4-galactosyltransferase, at 110,690 to 111,724
GFF395
GFF396 - Possible divergent polysaccharide deacetylase, at 111,711 to 112,673
GFF396
GFF397 - Periplasmic septal ring factor with murein hydrolase activity EnvC/YibP, at 112,677 to 113,960
GFF397
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-2
-1
0
1 at 110.803 kb on + strand, within GFF395 at 110.806 kb on + strand, within GFF395 at 110.996 kb on + strand, within GFF395 at 110.997 kb on - strand, within GFF395 at 110.997 kb on - strand, within GFF395 at 110.997 kb on - strand, within GFF395 at 111.013 kb on + strand, within GFF395 at 111.264 kb on + strand, within GFF395 at 111.265 kb on - strand, within GFF395 at 111.423 kb on + strand, within GFF395 at 111.587 kb on + strand, within GFF395 at 111.629 kb on + strand at 111.841 kb on + strand, within GFF396 at 111.842 kb on - strand, within GFF396 at 111.941 kb on + strand, within GFF396 at 111.942 kb on - strand, within GFF396 at 111.946 kb on - strand, within GFF396 at 111.946 kb on - strand, within GFF396 at 111.946 kb on - strand, within GFF396 at 112.240 kb on + strand, within GFF396 at 112.241 kb on - strand, within GFF396 at 112.241 kb on - strand, within GFF396 at 112.241 kb on - strand, within GFF396 at 112.382 kb on + strand, within GFF396 at 112.382 kb on + strand, within GFF396 at 112.463 kb on + strand, within GFF396 at 112.476 kb on - strand, within GFF396 at 112.513 kb on - strand, within GFF396 at 113.592 kb on - strand, within GFF397
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 110,803 + GFF395 0.11 -0.2 110,806 + GFF395 0.11 +0.2 110,996 + GFF395 0.30 -2.0 110,997 - GFF395 0.30 -0.3 110,997 - GFF395 0.30 +0.2 110,997 - GFF395 0.30 -1.4 111,013 + GFF395 0.31 +1.4 111,264 + GFF395 0.55 +0.1 111,265 - GFF395 0.56 -1.1 111,423 + GFF395 0.71 -0.5 111,587 + GFF395 0.87 -0.2 111,629 + +0.3 111,841 + GFF396 0.13 +0.5 111,842 - GFF396 0.14 +0.3 111,941 + GFF396 0.24 +0.5 111,942 - GFF396 0.24 +0.6 111,946 - GFF396 0.24 +0.6 111,946 - GFF396 0.24 -0.3 111,946 - GFF396 0.24 +0.8 112,240 + GFF396 0.55 +0.5 112,241 - GFF396 0.55 +0.7 112,241 - GFF396 0.55 -0.2 112,241 - GFF396 0.55 -0.0 112,382 + GFF396 0.70 -0.6 112,382 + GFF396 0.70 +0.2 112,463 + GFF396 0.78 +0.3 112,476 - GFF396 0.79 -0.3 112,513 - GFF396 0.83 -0.9 113,592 - GFF397 0.71 +0.1
Or see this region's nucleotide sequence